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10.1038/s41598-021-94095-7 http://scihub22266oqcxt.onion/10.1038/s41598-021-94095-7 34294757!8298517!34294757     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34294757&cmd=llinks): Failed to open stream: HTTP request failed! 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High-throughput human primary cell-based airway model for evaluating influenza, coronavirus, or other respiratory viruses in vitro |pdf=|usr=}}{{34294757}} gab.com Text High-throughput human primary cell-based airway model for evaluating influenza, coronavirus, or other respiratory viruses in vitro JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34294757 #FOAvip Influenza and other respiratory viruses present a significant threat to public health, national security, and the world economy, and can lead to the emergence of global pandemics such as from COVID-19. A barrier to the development of effective therapeutics is the absence of a robust and predictive preclinical model, with most studies relying on a combination of in vitro screening with immortalized cell lines and low-throughput animal models. Here, we integrate human primary airway epithelial cells into a custom-engineered 96-device platform (PREDICT96-ALI) in which tissues are cultured in an array of microchannel-based culture chambers at an air-liquid interface, in a configuration compatible with high resolution in-situ imaging and real-time sensing. We apply this platform to influenza A virus and coronavirus infections, evaluating viral infection kinetics and antiviral agent dosing across multiple strains and donor populations of human primary cells. Human coronaviruses HCoV-NL63 and SARS-CoV-2 enter host cells via ACE2 and utilize the protease TMPRSS2 for spike protein priming, and we confirm their expression, demonstrate infection across a range of multiplicities of infection, and evaluate the efficacy of camostat mesylate, a known inhibitor of HCoV-NL63 infection. This new capability can be used to address a major gap in the rapid assessment of therapeutic efficacy of small molecules and antiviral agents against influenza and other respiratory viruses including coronaviruses. Twit Text FOAVip High-throughput human primary cell-based airway model for evaluating influenza, coronavirus, or other respiratory viruses in vitro ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34294757 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34294757 #FOAvip English Wikipedia <ref>{{Cite pmid|34294757}}</ref> High-throughput human primary cell-based airway model for evaluating influenza, coronavirus, or other respiratory viruses in vitro #MMPMID34294757 Gard AL; Luu RJ; Miller CR; Maloney R; Cain BP; Marr EE; Burns DM; Gaibler R; Mulhern TJ; Wong CA; Alladina J; Coppeta JR; Liu P; Wang JP; Azizgolshani H; Fezzie RF; Balestrini JL; Isenberg BC; Medoff BD; Finberg RW; Borenstein JT Sci Rep 2021[Jul]; 11 (1): 14961 PMID34294757show ga Influenza and other respiratory viruses present a significant threat to public health, national security, and the world economy, and can lead to the emergence of global pandemics such as from COVID-19. A barrier to the development of effective therapeutics is the absence of a robust and predictive preclinical model, with most studies relying on a combination of in vitro screening with immortalized cell lines and low-throughput animal models. Here, we integrate human primary airway epithelial cells into a custom-engineered 96-device platform (PREDICT96-ALI) in which tissues are cultured in an array of microchannel-based culture chambers at an air-liquid interface, in a configuration compatible with high resolution in-situ imaging and real-time sensing. We apply this platform to influenza A virus and coronavirus infections, evaluating viral infection kinetics and antiviral agent dosing across multiple strains and donor populations of human primary cells. Human coronaviruses HCoV-NL63 and SARS-CoV-2 enter host cells via ACE2 and utilize the protease TMPRSS2 for spike protein priming, and we confirm their expression, demonstrate infection across a range of multiplicities of infection, and evaluate the efficacy of camostat mesylate, a known inhibitor of HCoV-NL63 infection. This new capability can be used to address a major gap in the rapid assessment of therapeutic efficacy of small molecules and antiviral agents against influenza and other respiratory viruses including coronaviruses. |Antiviral Agents/*pharmacology[MESH] |Bronchi/cytology/virology[MESH] |COVID-19 Drug Treatment[MESH] |COVID-19/virology[MESH] |Cell Culture Techniques/instrumentation[MESH] |Cell Line[MESH] |Coronavirus Infections/drug therapy/*virology[MESH] |Coronavirus/drug effects[MESH] |Equipment Design[MESH] |High-Throughput Screening Assays/instrumentation[MESH] |Humans[MESH] |Influenza A virus/drug effects[MESH] |Influenza, Human/drug therapy/*virology[MESH] |Microbial Sensitivity Tests/*instrumentation[MESH] |Microfluidic Analytical Techniques/*instrumentation[MESH] |Respiratory Mucosa/*cytology/virology[MESH] |Respiratory Tract Infections/drug therapy/virology[MESH]High-throughput human primary cell-based airway model for evaluating i(epmc).pdf DeepDyve Pubget Overpricing