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10.1016/j.coviro.2021.09.008 http://scihub22266oqcxt.onion/10.1016/j.coviro.2021.09.008 34601307!8504867!34601307     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34601307&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34601307.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Curr+Opin+Virol 2021 ; 51 (�): 74-79 Nephropedia Template TP {{tp|p=34601307|t=2021. Cryo-electron microscopy of nodavirus RNA replication organelles illuminates positive-strand RNA virus genome replication |pdf=|usr=}}{{34601307}} gab.com Text Cryo-electron microscopy of nodavirus RNA replication organelles illuminates positive-strand RNA virus genome replication JO: Curr Opin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34601307 #FOAvip The nodavirus flock house virus recently provided a well-characterized model for the first cryo-electron microscope tomography of membrane-bound, positive-strand RNA ((+)RNA) virus genome replication complexes (RCs). The resulting first views of RC organization and complementary biochemical results showed that the viral RNA replication vesicle is tightly packed with the dsRNA genomic RNA replication intermediate, and that (+)ssRNA replication products are released through the vesicle neck to the cytosol through a 12-fold symmetric ring or crown of multi-functional viral RNA replication proteins, which likely also contribute to viral RNA synthesis. Subsequent studies identified similar crown-like RNA replication protein complexes in alphavirus and coronavirus RCs, indicating related mechanisms across highly divergent (+)RNA viruses. As outlined in this review, these results have significant implications for viral function, evolution and control. Twit Text FOAVip Cryo-electron microscopy of nodavirus RNA replication organelles illuminates positive-strand RNA virus genome replication ????Curr Opin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34601307 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34601307 #FOAvip English Wikipedia <ref>{{Cite pmid|34601307}}</ref> Cryo-electron microscopy of nodavirus RNA replication organelles illuminates positive-strand RNA virus genome replication #MMPMID34601307 Unchwaniwala N; Zhan H; den Boon JA; Ahlquist P Curr Opin Virol 2021[Dec]; 51 (�): 74-79 PMID34601307show ga The nodavirus flock house virus recently provided a well-characterized model for the first cryo-electron microscope tomography of membrane-bound, positive-strand RNA ((+)RNA) virus genome replication complexes (RCs). The resulting first views of RC organization and complementary biochemical results showed that the viral RNA replication vesicle is tightly packed with the dsRNA genomic RNA replication intermediate, and that (+)ssRNA replication products are released through the vesicle neck to the cytosol through a 12-fold symmetric ring or crown of multi-functional viral RNA replication proteins, which likely also contribute to viral RNA synthesis. Subsequent studies identified similar crown-like RNA replication protein complexes in alphavirus and coronavirus RCs, indicating related mechanisms across highly divergent (+)RNA viruses. As outlined in this review, these results have significant implications for viral function, evolution and control. |Animals[MESH] |Cryoelectron Microscopy[MESH] |Nodaviridae/physiology/*ultrastructure[MESH] |Plants/virology[MESH] |RNA, Viral/ultrastructure[MESH]Cryo-electron microscopy of nodavirus RNA replication organelles illum(epmc).pdf DeepDyve Pubget Overpricing