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Protective effects elicited by cow milk fermented with L Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes #MMPMID34630633
Paparo L; Bruno C; Ferrucci V; Punto E; Viscardi M; Fusco G; Cerino P; Romano A; Zollo M; Berni Canani R
J Funct Foods 2021[Dec]; 87 (ä): 104787 PMID34630633show ga
Fermented foods have been proposed in limiting SARS-CoV-2 infection. Emerging evidence suggest the efficacy of cow's milk fermented with the probiotic L. paracasei CBAL74 (FM-CBAL74) in preventing infectious diseases. We evaluated the protective action of FM-CBAL74 against SARS-CoV-2 infection in human enterocytes. Relevant aspects of SARS-CoV-2 infection were assessed: infectivity, host functional receptor angiotensin-converting enzyme-2 (ACE2), transmembrane protease serine 2 (TMPRSS2), and pro-inflammatory cytokines expression (IL-6, IL-15, IL-1beta, VEGFbeta, TNF-alpha, MCP-1, CXCL1). Pre-incubation with FM-CBA L74 reduced the number of infected cells. The expression of ACE2 and the pro-inflammatory cytokines IL-6, VEGFbeta, IL-15, IL-1beta was downregulated by the pre-treatment with this fermented food. No effect on TMPRSS2, MCP-1, TNF-alpha and CXCL1 expression was observed. Modulating the crucial aspects of the infection, the fermented food FM-CBAL74 exerts a preventive action against SARS-CoV-2. These evidence could pave the way to innovative nutritional strategy to mitigate the COVID-19.