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10.1016/j.resmic.2021.103915 http://scihub22266oqcxt.onion/10.1016/j.resmic.2021.103915 34863883!8634697!34863883     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34863883&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34863883.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Res+Microbiol 2022 ; 173 (3): 103915 Nephropedia Template TP {{tp|p=34863883|t=2022. Fungal infections diagnosis - Past, present and future |pdf=|usr=}}{{34863883}} gab.com Text Fungal infections diagnosis - Past, present and future JO: Res Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34863883 #FOAvip Despite the scientific advances observed in the recent decades and the emergence of new methodologies, the diagnosis of systemic fungal infections persists as a problematic issue. Fungal cultivation, the standard method that allows a proven diagnosis, has numerous disadvantages, as low sensitivity (only 50% of the patients present positive fungal cultures), and long growth time. These are factors that delay the patient's treatment and, consequently, lead to higher hospital costs. To improve the accuracy and quickness of fungal infections diagnosis, several new methodologies attempt to be implemented in clinical microbiology laboratories. Most of these innovative methods are independent of pathogen isolation, which means that the diagnosis goes from being considered proven to probable. In spite of the advantage of being culture-independent, the majority of the methods lack standardization. PCR-based methods are becoming more and more commonly used, which has earned them an important place in hospital laboratories. This can be perceived now, as PCR-based methodologies have proved to be an essential tool fighting against the COVID-19 pandemic. This review aims to go through the main steps of the diagnosis for systemic fungal infection, from diagnostic classifications, through methodologies considered as "gold standard", to the molecular methods currently used, and finally mentioning some of the more futuristic approaches. Twit Text FOAVip Fungal infections diagnosis - Past, present and future ????Res Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34863883 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34863883 #FOAvip English Wikipedia <ref>{{Cite pmid|34863883}}</ref> Fungal infections diagnosis - Past, present and future #MMPMID34863883 Mendonca A; Santos H; Franco-Duarte R; Sampaio P Res Microbiol 2022[Mar]; 173 (3): 103915 PMID34863883show ga Despite the scientific advances observed in the recent decades and the emergence of new methodologies, the diagnosis of systemic fungal infections persists as a problematic issue. Fungal cultivation, the standard method that allows a proven diagnosis, has numerous disadvantages, as low sensitivity (only 50% of the patients present positive fungal cultures), and long growth time. These are factors that delay the patient's treatment and, consequently, lead to higher hospital costs. To improve the accuracy and quickness of fungal infections diagnosis, several new methodologies attempt to be implemented in clinical microbiology laboratories. Most of these innovative methods are independent of pathogen isolation, which means that the diagnosis goes from being considered proven to probable. In spite of the advantage of being culture-independent, the majority of the methods lack standardization. PCR-based methods are becoming more and more commonly used, which has earned them an important place in hospital laboratories. This can be perceived now, as PCR-based methodologies have proved to be an essential tool fighting against the COVID-19 pandemic. This review aims to go through the main steps of the diagnosis for systemic fungal infection, from diagnostic classifications, through methodologies considered as "gold standard", to the molecular methods currently used, and finally mentioning some of the more futuristic approaches. |*COVID-19/diagnosis[MESH] |*Mycoses/diagnosis[MESH] |Humans[MESH] |Pandemics[MESH]Fungal infections diagnosis - Past, present and future (epmc).pdf DeepDyve Pubget Overpricing