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10.2166/wh.2021.167 http://scihub22266oqcxt.onion/10.2166/wh.2021.167 35100156!ä!35100156 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\35100156.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Water+Health 2022 ; 20 (1): 83-91 Nephropedia Template TP {{tp|p=35100156|t=2022. SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae |pdf=|usr=}}{{35100156}} gab.com Text SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae JO: J Water Health http://www.kidney.de/pget.php?&tw=1&pmid=35100156 #FOAvip The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying the interaction of the enveloped bacteriophage Phi6, as a potential surrogated along with one of the most common amoebae in engineered aquatic environments, Vermamoeba vermiformis. Using microscopy, imaging flow cytometry and bacteriophage cell culture, our results imply that the SARS-CoV-2 surrogate triggers amoebic mitochondria and induced apoptosis to promote viral persistence in trophozoites. Furthermore, virus-infected amoebae were still infectious after 2 months within FLA cysts. These results suggest that amoebae could contribute to the environmental persistence of SARS-CoV-2, including disinfection processes. In addition, amoebae could be a successful model system for understanding respiratory virus-eukaryotic biology at the cellular and molecular levels. Twit Text FOAVip SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae ????J Water Health http://www.kidney.de/pget.php?&tw=1&pmid=35100156 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35100156 #FOAvip English Wikipedia <ref>{{Cite pmid|35100156}}</ref> SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae #MMPMID35100156 Dey R; Dlusskaya E; Ashbolt NJ J Water Health 2022[Jan]; 20 (1): 83-91 PMID35100156show ga The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying the interaction of the enveloped bacteriophage Phi6, as a potential surrogated along with one of the most common amoebae in engineered aquatic environments, Vermamoeba vermiformis. Using microscopy, imaging flow cytometry and bacteriophage cell culture, our results imply that the SARS-CoV-2 surrogate triggers amoebic mitochondria and induced apoptosis to promote viral persistence in trophozoites. Furthermore, virus-infected amoebae were still infectious after 2 months within FLA cysts. These results suggest that amoebae could contribute to the environmental persistence of SARS-CoV-2, including disinfection processes. In addition, amoebae could be a successful model system for understanding respiratory virus-eukaryotic biology at the cellular and molecular levels. |*Amoeba[MESH] |*Bacteriophages[MESH] |*COVID-19[MESH] |*Viruses[MESH] |Humans[MESH]SARS-CoV-2 surrogate (Phi6) environmental persistence within free-livi(epmc).pdf DeepDyve Pubget Overpricing