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10.1016/j.bbadis.2022.166496 http://scihub22266oqcxt.onion/10.1016/j.bbadis.2022.166496 35863591!9290338!35863591     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=35863591&cmd=llinks): Failed to open stream: HTTP request failed! 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SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis in proximal tubule epithelial cells |pdf=|usr=}}{{35863591}} gab.com Text SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis in proximal tubule epithelial cells JO: Biochim Biophys Acta Mol Basis Dis http://www.kidney.de/pget.php?&tw=1&pmid=35863591 #FOAvip Patients with COVID-19 have high prevalence of albuminuria which is used as a marker of progression of renal disease and is associated with severe COVID-19. We hypothesized that SARS-CoV-2 spike protein (S protein) could modulate albumin handling in proximal tubule epithelial cells (PTECs) and, consequently contribute to the albuminuria observed in patients with COVID-19. In this context, the possible effect of S protein on albumin endocytosis in PTECs was investigated. Two PTEC lines were used: HEK-293A and LLC-PK1. Incubation of both cell types with S protein for 16 h inhibited albumin uptake at the same magnitude. This effect was associated with canonical megalin-mediated albumin endocytosis because: (1) DQ-albumin uptake, a marker of the lysosomal degradation pathway, was reduced at a similar level compared with fluorescein isothiocyanate (FITC)-albumin uptake; (2) dextran-FITC uptake, a marker of fluid-phase endocytosis, was not changed; (3) cell viability and proliferation were not changed. The inhibitory effect of S protein on albumin uptake was only observed when it was added at the luminal membrane, and it did not involve the ACE2/Ang II/AT1R axis. Although both cells uptake S protein, it does not seem to be required for modulation of albumin endocytosis. The mechanism underlying the inhibition of albumin uptake by S protein encompasses a decrease in megalin expression without changes in megalin trafficking and stability. These results reveal a possible mechanism to explain the albuminuria observed in patients with COVID-19. Twit Text FOAVip SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis in proximal tubule epithelial cells ????Biochim Biophys Acta Mol Basis Dis http://www.kidney.de/pget.php?&tw=1&pmid=35863591 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=35863591 #FOAvip English Wikipedia <ref>{{Cite pmid|35863591}}</ref> SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis in proximal tubule epithelial cells #MMPMID35863591 Silva-Aguiar RP; Teixeira DE; Peruchetti DB; Florentino LS; Peres RAS; Gomes CP; Marzolo MP; Rocco PMR; Pinheiro AAS; Caruso-Neves C Biochim Biophys Acta Mol Basis Dis 2022[Dec]; 1868 (12): 166496 PMID35863591show ga Patients with COVID-19 have high prevalence of albuminuria which is used as a marker of progression of renal disease and is associated with severe COVID-19. We hypothesized that SARS-CoV-2 spike protein (S protein) could modulate albumin handling in proximal tubule epithelial cells (PTECs) and, consequently contribute to the albuminuria observed in patients with COVID-19. In this context, the possible effect of S protein on albumin endocytosis in PTECs was investigated. Two PTEC lines were used: HEK-293A and LLC-PK1. Incubation of both cell types with S protein for 16 h inhibited albumin uptake at the same magnitude. This effect was associated with canonical megalin-mediated albumin endocytosis because: (1) DQ-albumin uptake, a marker of the lysosomal degradation pathway, was reduced at a similar level compared with fluorescein isothiocyanate (FITC)-albumin uptake; (2) dextran-FITC uptake, a marker of fluid-phase endocytosis, was not changed; (3) cell viability and proliferation were not changed. The inhibitory effect of S protein on albumin uptake was only observed when it was added at the luminal membrane, and it did not involve the ACE2/Ang II/AT1R axis. Although both cells uptake S protein, it does not seem to be required for modulation of albumin endocytosis. The mechanism underlying the inhibition of albumin uptake by S protein encompasses a decrease in megalin expression without changes in megalin trafficking and stability. These results reveal a possible mechanism to explain the albuminuria observed in patients with COVID-19. |*COVID-19[MESH] |*Low Density Lipoprotein Receptor-Related Protein-2/metabolism[MESH] |Albumins/metabolism/pharmacology[MESH] |Albuminuria/metabolism[MESH] |Angiotensin-Converting Enzyme 2[MESH] |Cells, Cultured[MESH] |Dextrans/pharmacology[MESH] |Endocytosis/physiology[MESH] |Epithelial Cells/metabolism[MESH] |Fluorescein-5-isothiocyanate/metabolism/pharmacology[MESH] |Humans[MESH] |SARS-CoV-2[MESH]SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis(epmc).pdf DeepDyve Pubget Overpricing