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10.1113/expphysiol.1996.sp003941 http://scihub22266oqcxt.onion/10.1113/expphysiol.1996.sp003941 8737071!ä!8737071 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\8737071.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Exp+Physiol 1996 ; 81 (3): 367-74 Nephropedia Template TP {{tp|p=8737071|t=1996. Characterization of a sodium-dependent magnesium efflux from magnesium-loaded rat pancreatic acinar cells |pdf=|usr=}}{{8737071}} gab.com Text Characterization of a sodium-dependent magnesium efflux from magnesium-loaded rat pancreatic acinar cells JO: Exp Physiol http://www.kidney.de/pget.php?&tw=1&pmid=8737071 #FOAvip This study investigates the mechanism of magnesium (Mg2+) transport (efflux) from the exocrine rat pancreas. Permeabilized pancreatic acini were loaded with Mg2+ by employing a high-Mg2+ (12 mM) buffer containing A23187 (6 microM). Net Mg2+ efflux was measured using the technique of atomic absorbance spectrophotometry. Incubation of preloaded acini in a buffer deficient in Mg2+ resulted in a large and time-dependent release of Mg2+ with maximal efflux occurring within 40 min. Pretreatment of loaded acini with bumetanide, SITS or ouabain had no significant effect on Mg2+ efflux. In contrast, when acini were pretreated with 10 mM dinitrophenol, 10(-4) M amiloride, 1 mM lidocaine or 1 mM quinidine there were significant (P < 0.001) decreases in net Mg2+ efflux. Replacement of extracellular sodium [Na+]o with either N-methyl-D-glucamine (NMDG), Tris or choline resulted in a significant (P < 0.001) inhibition of Mg2+ efflux. The results of this study indicate that Mg2+ transport (efflux) in pancreatic acinar cells may not be associated with the Na(+)-K(+)-ATPase, the Na(+)-K(+)-Cl- cotransporter or the anion exchanger, but with a Na(2+)-sensitive Mg2+ transport system. Twit Text FOAVip Characterization of a sodium-dependent magnesium efflux from magnesium-loaded rat pancreatic acinar cells ????Exp Physiol http://www.kidney.de/pget.php?&tw=1&pmid=8737071 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=8737071 #FOAvip English Wikipedia <ref>{{Cite pmid|8737071}}</ref> Characterization of a sodium-dependent magnesium efflux from magnesium-loaded rat pancreatic acinar cells #MMPMID8737071 Wisdom DM; Geada MM; Singh J Exp Physiol 1996[May]; 81 (3): 367-74 PMID8737071show ga This study investigates the mechanism of magnesium (Mg2+) transport (efflux) from the exocrine rat pancreas. Permeabilized pancreatic acini were loaded with Mg2+ by employing a high-Mg2+ (12 mM) buffer containing A23187 (6 microM). Net Mg2+ efflux was measured using the technique of atomic absorbance spectrophotometry. Incubation of preloaded acini in a buffer deficient in Mg2+ resulted in a large and time-dependent release of Mg2+ with maximal efflux occurring within 40 min. Pretreatment of loaded acini with bumetanide, SITS or ouabain had no significant effect on Mg2+ efflux. In contrast, when acini were pretreated with 10 mM dinitrophenol, 10(-4) M amiloride, 1 mM lidocaine or 1 mM quinidine there were significant (P < 0.001) decreases in net Mg2+ efflux. Replacement of extracellular sodium [Na+]o with either N-methyl-D-glucamine (NMDG), Tris or choline resulted in a significant (P < 0.001) inhibition of Mg2+ efflux. The results of this study indicate that Mg2+ transport (efflux) in pancreatic acinar cells may not be associated with the Na(+)-K(+)-ATPase, the Na(+)-K(+)-Cl- cotransporter or the anion exchanger, but with a Na(2+)-sensitive Mg2+ transport system. |4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/pharmacology[MESH] |Amiloride/pharmacology[MESH] |Animals[MESH] |Biological Transport[MESH] |Bumetanide/pharmacology[MESH] |Choline/pharmacology[MESH] |Dinitrophenols/pharmacology[MESH] |Female[MESH] |In Vitro Techniques[MESH] |Lidocaine/pharmacology[MESH] |Magnesium/*metabolism/pharmacology[MESH] |Male[MESH] |Ouabain/pharmacology[MESH] |Pancreas/drug effects/*metabolism[MESH] |Quinidine/pharmacology[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH]Characterization of a sodium-dependent magnesium efflux from magnesium(epmc).pdf DeepDyve Pubget Overpricing