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10.1152/ajpcell.1996.271.5.C1705 http://scihub22266oqcxt.onion/10.1152/ajpcell.1996.271.5.C1705 8944655!ä!8944655 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=8944655&cmd=llinks): Failed to open stream: HTTP request failed! 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Angiotensin II activation of Ca(2+)-permeant nonselective cation channels in rat adrenal glomerulosa cells |pdf=|usr=}}{{8944655}} gab.com Text Angiotensin II activation of Ca(2+)-permeant nonselective cation channels in rat adrenal glomerulosa cells JO: Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=8944655 #FOAvip A Ca(2+)-permeant, nonselective cation channel was observed in cell-attached and inside-out membrane patches from rat adrenal glomerulosa cells maintained in primary cell culture. In cell-attached patches under near physiological ionic conditions, single-channel currents exhibited a reversal potential near -10 mV, inward rectification, a nearly linear slope conductance between 0 and -80 mV of 17.4 pS, and voltage-dependent block at potentials more negative than -80 mV. Channels exhibiting identical conductance and gating properties were observed in inside-out patches; however, channel gating ran down within minutes in this configuation. In the inside-out configuration, channel gating did not require cytosolic Ca2+ (Ca2+ < 10(-9) M), and inward rectification was relieved by removal of intracellular Mg2+. Relative ionic permeability was calculated using reversal potential measurements from inside-out patches under bi-ionic conditions. The channel discriminated poorly among monovalent cations (PLi > PK > PCs > PNa) and was not significantly permeable to anions. The channel was permeable to Ca2+, exhibiting a relative permeability ratio of 0.29 PCa/PNa) when measured with 110 mM Ca2+ on the intracellular face and a permeability ratio of 4.38 (PCa/PNa) with 110 mM Ca2+ on the extracellular face. Channel gating behavior was episodic with open times ranging from milliseconds to tens of seconds and closed times lasting up to several minutes or longer. Channel gating appeared to be relatively voltage independent except that mean channel open time and open probability were reduced by membrane hyperpolarization. In cell-attached patches, bath application of 1 nM angiotensin II (ANG II) increased the channel open probability, primarily affecting channels exhibiting a low open probability, primarily affecting channels exhibiting a low open probability before stimulation. With the use of nystatin perforated-patch current clamp to measure membrane potential, ANG II was observed to induce large transient membrane depolarizations, consistent with activation of an inward current. We hypothesize that this channel is an important component of ANG II-induced membrane depolarization and Ca2+ influx during stimulation of aldosterone secretion. Twit Text FOAVip Angiotensin II activation of Ca(2+)-permeant nonselective cation channels in rat adrenal glomerulosa cells ????Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=8944655 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=8944655 #FOAvip English Wikipedia <ref>{{Cite pmid|8944655}}</ref> Angiotensin II activation of Ca(2+)-permeant nonselective cation channels in rat adrenal glomerulosa cells #MMPMID8944655 Lotshaw DP; Li F Am J Physiol 1996[Nov]; 271 (5 Pt 1): C1705-15 PMID8944655show ga A Ca(2+)-permeant, nonselective cation channel was observed in cell-attached and inside-out membrane patches from rat adrenal glomerulosa cells maintained in primary cell culture. In cell-attached patches under near physiological ionic conditions, single-channel currents exhibited a reversal potential near -10 mV, inward rectification, a nearly linear slope conductance between 0 and -80 mV of 17.4 pS, and voltage-dependent block at potentials more negative than -80 mV. Channels exhibiting identical conductance and gating properties were observed in inside-out patches; however, channel gating ran down within minutes in this configuation. In the inside-out configuration, channel gating did not require cytosolic Ca2+ (Ca2+ < 10(-9) M), and inward rectification was relieved by removal of intracellular Mg2+. Relative ionic permeability was calculated using reversal potential measurements from inside-out patches under bi-ionic conditions. The channel discriminated poorly among monovalent cations (PLi > PK > PCs > PNa) and was not significantly permeable to anions. The channel was permeable to Ca2+, exhibiting a relative permeability ratio of 0.29 PCa/PNa) when measured with 110 mM Ca2+ on the intracellular face and a permeability ratio of 4.38 (PCa/PNa) with 110 mM Ca2+ on the extracellular face. Channel gating behavior was episodic with open times ranging from milliseconds to tens of seconds and closed times lasting up to several minutes or longer. Channel gating appeared to be relatively voltage independent except that mean channel open time and open probability were reduced by membrane hyperpolarization. In cell-attached patches, bath application of 1 nM angiotensin II (ANG II) increased the channel open probability, primarily affecting channels exhibiting a low open probability, primarily affecting channels exhibiting a low open probability before stimulation. With the use of nystatin perforated-patch current clamp to measure membrane potential, ANG II was observed to induce large transient membrane depolarizations, consistent with activation of an inward current. We hypothesize that this channel is an important component of ANG II-induced membrane depolarization and Ca2+ influx during stimulation of aldosterone secretion. |Angiotensin II/*pharmacology[MESH] |Animals[MESH] |Calcium/*metabolism[MESH] |Cell Membrane/physiology[MESH] |Cells, Cultured[MESH] |Cytosol/metabolism[MESH] |Egtazic Acid/pharmacology[MESH] |Female[MESH] |Ion Channel Gating[MESH] |Ion Channels/*physiology[MESH] |Kinetics[MESH] |Magnesium/metabolism[MESH] |Membrane Potentials/drug effects[MESH] |Patch-Clamp Techniques[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH] |Sodium/metabolism[MESH] |Substrate Specificity[MESH] |Time Factors[MESH]Angiotensin II activation of Ca(2+)-permeant nonselective cation chann(epmc).pdf DeepDyve Pubget Overpricing