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10.1080/15513819609168684 http://scihub22266oqcxt.onion/10.1080/15513819609168684 9025846!�!9025846 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=9025846&cmd=llinks): Failed to open stream: HTTP request failed! 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Polymerase chain reaction amplification of archival material for parvovirus B19 in children with transient erythroblastopenia of childhood |pdf=|usr=}}{{9025846}} gab.com Text Polymerase chain reaction amplification of archival material for parvovirus B19 in children with transient erythroblastopenia of childhood JO: Pediatr Pathol Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=9025846 #FOAvip The relationship between transient erythroblastopenia of childhood (TEC) and parvovirus B19 infection remains uncertain. Large series using primarily serologic evaluation have not shown an association, whereas smaller series have reported parvovirus B19 infection in such patients. Further, parvovirus DNA or antigen has been detected in some patients seronegative for the virus at presentation. Polymerase chain reaction (PCR) amplification has never been used to evaluate patients with TEC for parvovirus B19. We used the PCR in an attempt to detect parvovirus B19 in DNA extracted from archived bone marrow coverslips of 16 patients diagnosed with TEC. The patients ranged in age from 3 to 23 months and presented with a mean hemoglobin value of 5.4 g/dL. Sixty-nine percent were neutropenic and none was thrombocytopenic. None of the patients had histologic evidence of parvovirus B19 infection in the bone marrow. DNA amplification for parvovirus B19 was negative in each case. In contrast, parvovirus B19DNA was amplified from DNA isolated from archived bone marrow coverslips of a patient with known parvovirus B19 infection, indicating that the PCR assay was sufficiently sensitive to detect virus from archieved bone marrow coverslips. Review of the literature indicates that the patients with parvovirus-associated TEC are generally older and often present with concomitant thrombocytopenia, whereas patients with parvovirus B19-negative TEC are younger and present without thrombocytopenia, similar to the patients in our study. Our results suggest that parvovirus B19 is not the cause of anemia in the young patient with typical features of TEC. Rather, parvovirus B19 infection of older, previously healthy children may occasionally cause a protracted anemia, often with thrombocytopenia, which may be diagnosed by some as TEC. Twit Text FOAVip Polymerase chain reaction amplification of archival material for parvovirus B19 in children with transient erythroblastopenia of childhood ????Pediatr Pathol Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=9025846 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9025846 #FOAvip English Wikipedia <ref>{{Cite pmid|9025846}}</ref> Polymerase chain reaction amplification of archival material for parvovirus B19 in children with transient erythroblastopenia of childhood #MMPMID9025846 Rogers BB; Rogers ZR; Timmons CF Pediatr Pathol Lab Med 1996[May]; 16 (3): 471-8 PMID9025846show ga The relationship between transient erythroblastopenia of childhood (TEC) and parvovirus B19 infection remains uncertain. Large series using primarily serologic evaluation have not shown an association, whereas smaller series have reported parvovirus B19 infection in such patients. Further, parvovirus DNA or antigen has been detected in some patients seronegative for the virus at presentation. Polymerase chain reaction (PCR) amplification has never been used to evaluate patients with TEC for parvovirus B19. We used the PCR in an attempt to detect parvovirus B19 in DNA extracted from archived bone marrow coverslips of 16 patients diagnosed with TEC. The patients ranged in age from 3 to 23 months and presented with a mean hemoglobin value of 5.4 g/dL. Sixty-nine percent were neutropenic and none was thrombocytopenic. None of the patients had histologic evidence of parvovirus B19 infection in the bone marrow. DNA amplification for parvovirus B19 was negative in each case. In contrast, parvovirus B19DNA was amplified from DNA isolated from archived bone marrow coverslips of a patient with known parvovirus B19 infection, indicating that the PCR assay was sufficiently sensitive to detect virus from archieved bone marrow coverslips. Review of the literature indicates that the patients with parvovirus-associated TEC are generally older and often present with concomitant thrombocytopenia, whereas patients with parvovirus B19-negative TEC are younger and present without thrombocytopenia, similar to the patients in our study. Our results suggest that parvovirus B19 is not the cause of anemia in the young patient with typical features of TEC. Rather, parvovirus B19 infection of older, previously healthy children may occasionally cause a protracted anemia, often with thrombocytopenia, which may be diagnosed by some as TEC. |Child, Preschool[MESH] |DNA Replication/*genetics[MESH] |DNA, Viral/*analysis[MESH] |Female[MESH] |Humans[MESH] |Infant[MESH] |Male[MESH] |Parvovirus B19, Human/*isolation & purification[MESH] |Polymerase Chain Reaction/methods[MESH] |Red-Cell Aplasia, Pure/*virology[MESH]Polymerase chain reaction amplification of archival material for parvo(epmc).pdf DeepDyve Pubget Overpricing