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10.1152/ajpgi.1998.275.5.G1106 http://scihub22266oqcxt.onion/10.1152/ajpgi.1998.275.5.G1106 9815041!�!9815041 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=9815041&cmd=llinks): Failed to open stream: HTTP request failed! 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Acute effect of EtOH on Mg2+ homeostasis in liver cells: evidence for the activation of an Na+/Mg2+ exchanger |pdf=|usr=}}{{9815041}} gab.com Text Acute effect of EtOH on Mg2+ homeostasis in liver cells: evidence for the activation of an Na+/Mg2+ exchanger JO: Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9815041 #FOAvip The acute administration of ethanol mobilizes a considerable amount of Mg2+ from perfused rat livers and isolated hepatocytes in a dose-dependent fashion in the absence of release of cellular K+ or lactate dehydrogenase (LDH) in the extracellular medium. Mg2+ extrusion becomes detectable within 2 min and reaches the maximum within 8 min after ethanol addition, declining toward the basal value thereafter irrespective of the persistence of alcohol in the perfusion system and the dose of ethanol administered. The effect is the result of a specific impairment of Mg2+ transport and/or regulatory mechanisms. In fact, Mg2+ extrusion does not occur under conditions in which 1) ethanol is replaced by an equivalent dose of DMSO, 2) amiloride or imipramine are used as inhibitors of the Na+/Mg2+ exchanger, 3) extracellular Na+ is replaced by an equimolar concentration of choline chloride, and 4) 4-methylpyrazole is used to specifically inhibit alcohol dehydrogenase and cytochrome P-4502E1. Finally, the observation that the cellular level of ATP is markedly reduced after acute ethanol administration would suggest that Mg2+ extrusion results from a decreased buffering capacity of cytosolic Mg-ATP complex. Twit Text FOAVip Acute effect of EtOH on Mg2+ homeostasis in liver cells: evidence for the activation of an Na+/Mg2+ exchanger ????Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=9815041 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=9815041 #FOAvip English Wikipedia <ref>{{Cite pmid|9815041}}</ref> Acute effect of EtOH on Mg2+ homeostasis in liver cells: evidence for the activation of an Na+/Mg2+ exchanger #MMPMID9815041 Tessman PA; Romani A Am J Physiol 1998[Nov]; 275 (5): G1106-16 PMID9815041show ga The acute administration of ethanol mobilizes a considerable amount of Mg2+ from perfused rat livers and isolated hepatocytes in a dose-dependent fashion in the absence of release of cellular K+ or lactate dehydrogenase (LDH) in the extracellular medium. Mg2+ extrusion becomes detectable within 2 min and reaches the maximum within 8 min after ethanol addition, declining toward the basal value thereafter irrespective of the persistence of alcohol in the perfusion system and the dose of ethanol administered. The effect is the result of a specific impairment of Mg2+ transport and/or regulatory mechanisms. In fact, Mg2+ extrusion does not occur under conditions in which 1) ethanol is replaced by an equivalent dose of DMSO, 2) amiloride or imipramine are used as inhibitors of the Na+/Mg2+ exchanger, 3) extracellular Na+ is replaced by an equimolar concentration of choline chloride, and 4) 4-methylpyrazole is used to specifically inhibit alcohol dehydrogenase and cytochrome P-4502E1. Finally, the observation that the cellular level of ATP is markedly reduced after acute ethanol administration would suggest that Mg2+ extrusion results from a decreased buffering capacity of cytosolic Mg-ATP complex. |Adenosine Diphosphate/metabolism[MESH] |Adenosine Triphosphate/metabolism[MESH] |Amiloride/pharmacology[MESH] |Animals[MESH] |Antiporters/*metabolism[MESH] |Cells, Cultured[MESH] |Cyclic AMP/metabolism[MESH] |Ethanol/*pharmacology[MESH] |Homeostasis/*drug effects[MESH] |Kinetics[MESH] |L-Lactate Dehydrogenase/analysis[MESH] |Liver/drug effects/*metabolism[MESH] |Magnesium/*metabolism[MESH] |Male[MESH] |Models, Biological[MESH] |Potassium/metabolism[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH]Acute effect of EtOH on Mg2+ homeostasis in liver cells: evidence for (epmc).pdf DeepDyve Pubget Overpricing