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10.1309/AJCPN4L1BMRQPEIT

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C3662488!3662488!23690120
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suck abstract from ncbi


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pmid23690120      Am+J+Clin+Pathol 2013 ; 139 (6): 771-9
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  • Utility of a Monoclonal ERG/FLI1 Antibody for Immunohistochemical Discrimination of Ewing?s Family Tumors #MMPMID23690120
  • Tomlins SA; Palanisamy N; Brenner JC; Stall JN; Siddiqui J; Thomas DG; Lucas DR; Chinnaiyan AM; Kunju LP
  • Am J Clin Pathol 2013[Jun]; 139 (6): 771-9 PMID23690120show ga
  • Ewing family tumors (EFTs) and prostate carcinomas (PCa) are characterized by rearrangement of ETS genes, most commonly FLI1 (EFTs) and ERG (PCa). Previously, we characterized an antibody against ERG (EPR3864) for detecting ERG-rearranged PCa. EPR3864 also cross reacts with FLI1, thus, here we evaluated the utility of EPR3864 for discriminating EFTs from other small round blue cell tumors (SRBCTs) by immunohistochemistry. Of 57 evaluable EFTs, 47 (82%) demonstrated at least moderate, diffuse, nuclear ERG/FLI1 staining (including 89% and 100% of cases with confirmed EWSR1:FLI1 and EWSR1:ERG fusions, respectively), of which 1, 3 and 43 showed negative, cytoplasmic or membranous CD99 staining, respectively. Amongst other SRBCTs (n=61 cases, 6 types), at least moderate, diffuse, nuclear EPR3864 staining was seen in all precursor-B-lymphoblastic lymphomas/leukemias and subsets of Burkitt?s lymphomas (10%) and synovial sarcomas (45%). In summary, EPR3864 may have utility for detecting EWSR1:FLI1 and EWSR1:ERG rearranged EFTs, in addition to PCa.
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