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10.1093/dnares/dsu043

http://scihub22266oqcxt.onion/10.1093/dnares/dsu043
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suck abstract from ncbi


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pmid25428893      DNA+Res 2015 ; 22 (1): 101-7
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  • A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster #MMPMID25428893
  • Iguchi A; Iyoda S; Kikuchi T; Ogura Y; Katsura K; Ohnishi M; Hayashi T; Thomson NR
  • DNA Res 2015[Feb]; 22 (1): 101-7 PMID25428893show ga
  • The O antigen constitutes the outermost part of the lipopolysaccharide layer in Gram-negative bacteria. The chemical composition and structure of the O antigen show high levels of variation even within a single species revealing itself as serological diversity. Here, we present a complete sequence set for the O-antigen biosynthesis gene clusters (O-AGCs) from all 184 recognized Escherichia coli O serogroups. By comparing these sequences, we identified 161 well-defined O-AGCs. Based on the wzx/wzy or wzm/wzt gene sequences, in addition to 145 singletons, 37 serogroups were placed into 16 groups. Furthermore, phylogenetic analysis of all the E. coli O-serogroup reference strains revealed that the nearly one-quarter of the 184 serogroups were found in the ST10 lineage, which may have a unique genetic background allowing a more successful exchange of O-AGCs. Our data provide a complete view of the genetic diversity of O-AGCs in E. coli showing a stronger association between host phylogenetic lineage and O-serogroup diversification than previously recognized. These data will be a valuable basis for developing a systematic molecular O-typing scheme that will allow traditional typing approaches to be linked to genomic exploration of E. coli diversity.
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