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10.1111/cas.12667 http://scihub22266oqcxt.onion/10.1111/cas.12667 C4471796!4471796!25865675     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25865675&cmd=llinks): Failed to open stream: HTTP request failed! 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Time-lapse imaging assay using the BioStation CT: A�sensitive drug-screening method for three-dimensional cell culture |pdf=|usr=}}{{25865675}} gab.com Text Time-lapse imaging assay using the BioStation CT: A�sensitive drug-screening method for three-dimensional cell culture JO: Cancer Sci http://www.kidney.de/pget.php?&tw=1&pmid=25865675 #FOAvip Three-dimensional (3D) cell culture is beneficial for physiological studies of tumor cells, due to its potential to deliver a high quantity of cell culture information that is representative of the cancer microenvironment and predictive of drug responses in�vivo. Currently, gel-associated or matrix-associated 3D cell culture is comprised of intricate procedures that often result in experimental complexity. Therefore, we developed an innovative anti-cancer drug sensitivity screening technique for 3D cell culture on NanoCulture Plates (NCP) by employing the imaging device BioStation CT. Here, we showed that the human breast cancer cell lines BT474 and T47D form multicellular spheroids on NCP plates and compared their sensitivity to the anti-cancer drugs trastuzumab and paclitaxel using the BioStation CT. The anticancer drugs reduced spheroid migration velocity and suppressed spheroid fusion. In addition, primary cells derived from the human breast cancer tissues B58 and B61 grown on NCP plates also exhibited similar drug sensitivity. These results were in good agreement with the conventional assay method using ATP quantification. We confirmed the antitumor effects of the drugs on cells seeded in 96-well plates using the BioStation CT imaging technique. We expect this method to be useful in research for new antitumor agents and for drug sensitivity tests in individually-tailored cancer treatments. Twit Text FOAVip Time-lapse imaging assay using the BioStation CT: A�sensitive drug-screening method for three-dimensional cell culture ????Cancer Sci http://www.kidney.de/pget.php?&tw=1&pmid=25865675 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25865675 #FOAvip English Wikipedia <ref>{{Cite pmid|25865675}}</ref> Time-lapse imaging assay using the BioStation CT: A�sensitive drug-screening method for three-dimensional cell culture #MMPMID25865675 Sakamoto R; Rahman MM; Shimomura M; Itoh M; Nakatsura T Cancer Sci 2015[Jun]; 106 (6): 757-65 PMID25865675show ga Three-dimensional (3D) cell culture is beneficial for physiological studies of tumor cells, due to its potential to deliver a high quantity of cell culture information that is representative of the cancer microenvironment and predictive of drug responses in�vivo. Currently, gel-associated or matrix-associated 3D cell culture is comprised of intricate procedures that often result in experimental complexity. Therefore, we developed an innovative anti-cancer drug sensitivity screening technique for 3D cell culture on NanoCulture Plates (NCP) by employing the imaging device BioStation CT. Here, we showed that the human breast cancer cell lines BT474 and T47D form multicellular spheroids on NCP plates and compared their sensitivity to the anti-cancer drugs trastuzumab and paclitaxel using the BioStation CT. The anticancer drugs reduced spheroid migration velocity and suppressed spheroid fusion. In addition, primary cells derived from the human breast cancer tissues B58 and B61 grown on NCP plates also exhibited similar drug sensitivity. These results were in good agreement with the conventional assay method using ATP quantification. We confirmed the antitumor effects of the drugs on cells seeded in 96-well plates using the BioStation CT imaging technique. We expect this method to be useful in research for new antitumor agents and for drug sensitivity tests in individually-tailored cancer treatments. �Time-lapse imaging assay using the BioStation CT: A�sensitive drug-scr(epmc).pdf DeepDyve Pubget Overpricing