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Neuroprotective effect of rapamycin on spinal cord injury via activation of the Wnt/?-catenin signaling pathway #MMPMID26199613
Gao K; Wang Ys; Yuan Yj; Wan Zh; Yao Tc; Li Hh; Tang Pf; Mei Xf
Neural Regen Res 2015[Jun]; 10 (6): 951-7 PMID26199613show ga
The Wnt/?-catenin signaling pathway plays a crucial role in neural development, axonal guidance, neuropathic pain remission and neuronal survival. In this study, we initially examined the effect of rapamycin on the Wnt/?-catenin signaling pathway after spinal cord injury, by intraperitoneally injecting spinal cord injured rats with rapamycin over 2 days. Western blot analysis and immunofluorescence staining were used to detect the expression levels of ?-catenin protein, caspase-3 protein and brain-derived neurotrophic factor protein, components of the Wnt/?-catenin signaling pathway. Rapamycin increased the levels of ?-catenin and brain-derived neurotrophic factor in the injured spinal cord, improved the pathological morphology at the injury site, reduced the loss of motor neurons, and promoted motor functional recovery in rats after spinal cord injury. Our experimental findings suggest that the neuroprotective effect of rapamycin intervention is mediated through activation of the Wnt/?-catenin signaling pathway after spinal cord injury.