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C4563995!4563995!4005843
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pmid4005843      Cancer+Res 1985 ; 45 (7): 3053-7
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  • Biological and Biophysical Properties of the Tumor-localizing Component of Hematoporphyrin Derivative1 #MMPMID4005843
  • Kessel D; Cheng ML
  • Cancer Res 1985[Jul]; 45 (7): 3053-7 PMID4005843show ga
  • Reverse-phase chromatography, aqueous gel exclusion, and nonaqueous gel exclusion were assessed as procedures for preparative fractionation of the tumor-localizing product hematoporphyrin derivative. Porphyrin accumulation, fluorescence, and photodynamic cytotoxicity were monitored using the murine Sarcoma 180 tumor. Aqueous gel exclusion chromatography can provide a hematoporphyrin derivative fraction enriched in the tumor-localizing component. A further enrichment occurs when this procedure is carried out at 55�C, but nonlocalizing porphyrins could not be eliminated. While providing a better separation, reverse-phase chromatography cannot provide a tumor-localizing fraction free from contaminating protoporphyrin. However, this and other contaminants can be eliminated from the tumor-localizing fraction via nonaqueous gel exclusion chromatography. This latter separation provides two tumor-localizing products: (a) a fast-eluting fraction enriched in the major photosensitizing component(s); and (b) a more complex slowly eluting fraction enriched in fluorescence localizers.



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