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10.1038/srep14982

http://scihub22266oqcxt.onion/10.1038/srep14982
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C4595735!4595735!26443231
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suck abstract from ncbi


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pmid26443231      Sci+Rep 2015 ; 5 (�): �
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  • Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in rats using synchrotron radiation #MMPMID26443231
  • Zhang MQ; Zhou L; Deng QF; Xie YY; Xiao TQ; Cao YZ; Zhang JW; Chen XM; Yin XZ; Xiao B
  • Sci Rep 2015[]; 5 (�): � PMID26443231show ga
  • The angioarchitecture is a fundamental aspect of brain development and physiology. However, available imaging tools are unsuited for non-destructive cerebral mapping of the functionally important three-dimensional (3D) vascular microstructures. To address this issue, we developed an ultra-high resolution 3D digitalized angioarchitectural map for rat brain, based on synchrotron radiation phase contrast imaging (SR-PCI) with pixel size of 5.92??m. This approach provides a systematic and detailed view of the cerebrovascular anatomy at the micrometer level without any need for contrast agents. From qualitative and quantitative perspectives, the present 3D data provide a considerable insight into the spatial vascular network for whole rodent brain, particularly for functionally important regions of interest, such as the hippocampus, pre-frontal cerebral cortex and the corpus striatum. We extended these results to synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of targeted vessels in 3D. The SR-PCI method for systematic visualization of cerebral microvasculature holds considerable promise for wider application in life sciences, including 3D micro-imaging in experimental models of neurodevelopmental and vascular disorders.



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