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10.1186/s13075-015-0811-2 http://scihub22266oqcxt.onion/10.1186/s13075-015-0811-2 C4618946!4618946!26490351     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26490351.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Arthritis+Res+Ther 2015 ; 17 (ä): ä Nephropedia Template TP {{tp|p=26490351|t=2015. Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy |pdf=|usr=}}{{26490351}} gab.com Text Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy JO: Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=26490351 #FOAvip Introduction: While protective plasma cells (PCs) are an important part of the individual?s immune defense, autoreactive plasma cells such as dsDNA-specific plasma cells contribute to the pathogenesis of autoimmune diseases like systemic lupus erythematosus (SLE). However, the research on dsDNA-specific plasma cells was restricted to the ELISpot technique, with its limitations, as no other attempt for identification of dsDNA-reactive plasma cells had been successful. Methods: With improved fluorochrome labeling of dsDNA, removal of DNA aggregates, and enhanced blocking of unspecific binding, we were able to specifically detect dsDNA-reactive plasma cells by immunofluorescence microscopy. Results: Via this novel technique we were able to distinguish short-lived (SLPCs) and long-lived (LLPCs) autoreactive plasma cells, discriminate dsDNA-specific plasma cells according to their immunoglobulin class (IgG, IgM, and IgA) and investigate autoreactive (dsDNA) and vaccine-induced ovalbumin (Ova) plasma cells in parallel. Conclusions: The detection of autoreactive dsDNA-specific plasma cells via immunofluorescence microscopy allows specific studies on pathogenic and protective plasma cell subsets and their niches, detailed evaluation of therapeutic treatments and therefore offers new possibilities for basic and clinical research. Electronic supplementary material: The online version of this article (doi:10.1186/s13075-015-0811-2) contains supplementary material, which is available to authorized users. Twit Text FOAVip Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy ????Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=26490351 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26490351 #FOAvip English Wikipedia <ref>{{Cite pmid|26490351}}</ref> Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy #MMPMID26490351 Winter O; Musiol S; Schablowsky M; Cheng Q; Khodadadi L; Hiepe F Arthritis Res Ther 2015[]; 17 (ä): ä PMID26490351show ga Introduction: While protective plasma cells (PCs) are an important part of the individual?s immune defense, autoreactive plasma cells such as dsDNA-specific plasma cells contribute to the pathogenesis of autoimmune diseases like systemic lupus erythematosus (SLE). However, the research on dsDNA-specific plasma cells was restricted to the ELISpot technique, with its limitations, as no other attempt for identification of dsDNA-reactive plasma cells had been successful. Methods: With improved fluorochrome labeling of dsDNA, removal of DNA aggregates, and enhanced blocking of unspecific binding, we were able to specifically detect dsDNA-reactive plasma cells by immunofluorescence microscopy. Results: Via this novel technique we were able to distinguish short-lived (SLPCs) and long-lived (LLPCs) autoreactive plasma cells, discriminate dsDNA-specific plasma cells according to their immunoglobulin class (IgG, IgM, and IgA) and investigate autoreactive (dsDNA) and vaccine-induced ovalbumin (Ova) plasma cells in parallel. Conclusions: The detection of autoreactive dsDNA-specific plasma cells via immunofluorescence microscopy allows specific studies on pathogenic and protective plasma cell subsets and their niches, detailed evaluation of therapeutic treatments and therefore offers new possibilities for basic and clinical research. Electronic supplementary material: The online version of this article (doi:10.1186/s13075-015-0811-2) contains supplementary material, which is available to authorized users. äAnalyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells (epmc).pdf DeepDyve Pubget Overpricing