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10.1186/s13071-015-1197-3 http://scihub22266oqcxt.onion/10.1186/s13071-015-1197-3 C4640404!4640404!26552582     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26552582&cmd=llinks): Failed to open stream: HTTP request failed! 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Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro |pdf=|usr=}}{{26552582}} gab.com Text Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro JO: Parasit Vectors http://www.kidney.de/pget.php?&tw=1&pmid=26552582 #FOAvip Background: Hepatic myofibroblasts are relevant for pathogenesis of S. mansoni infection. In normal liver, these perisinusoidal cells are quiescent, express the lipocyte phenotype, and are located in the Disse?s space, being the major site of vitamin A storage. When activated, they convert to myofibroblasts and contribute to granulomatous and diffuse liver fibrosis. In the present work, we observed that myofibroblasts obtained from granulomatous periovular inflammatory reactions in schistosome-infected mice (GR-MF) produce in vitro immunomodulatory cytokines for eosinophil activation: IL-5 and eotaxin. Methods and results: The secretory activity of GR-MF was detected after TGF-? and IL-13 stimulation using 2D and 3D cell culture systems. In a mixed co-culture system using GR-MF with hematopoietic bone marrow cells from infected mice, we observed eosinophil survival that was dependent upon IL-5 and eotaxin, since antibodies against this cytokines decreased eosinophil population, as measured by eosinophil peroxidase activity. Conclusion: These results indicate that GR-MF may contribute to maintenance of local eosinophilia in schistosomal hepatic granulomas, and can function as immunoregulatory cells, besides their role in production of fibrosis. Electronic supplementary material: The online version of this article (doi:10.1186/s13071-015-1197-3) contains supplementary material, which is available to authorized users. Twit Text FOAVip Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro ????Parasit Vectors http://www.kidney.de/pget.php?&tw=1&pmid=26552582 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26552582 #FOAvip English Wikipedia <ref>{{Cite pmid|26552582}}</ref> Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro #MMPMID26552582 Paiva LA; Brand C; Bandeira-Melo C; Bozza PT; El-Cheikh MC; Silva PM; Borojevic R; Perez SAC Parasit Vectors 2015[]; 8 (�): � PMID26552582show ga Background: Hepatic myofibroblasts are relevant for pathogenesis of S. mansoni infection. In normal liver, these perisinusoidal cells are quiescent, express the lipocyte phenotype, and are located in the Disse?s space, being the major site of vitamin A storage. When activated, they convert to myofibroblasts and contribute to granulomatous and diffuse liver fibrosis. In the present work, we observed that myofibroblasts obtained from granulomatous periovular inflammatory reactions in schistosome-infected mice (GR-MF) produce in vitro immunomodulatory cytokines for eosinophil activation: IL-5 and eotaxin. Methods and results: The secretory activity of GR-MF was detected after TGF-? and IL-13 stimulation using 2D and 3D cell culture systems. In a mixed co-culture system using GR-MF with hematopoietic bone marrow cells from infected mice, we observed eosinophil survival that was dependent upon IL-5 and eotaxin, since antibodies against this cytokines decreased eosinophil population, as measured by eosinophil peroxidase activity. Conclusion: These results indicate that GR-MF may contribute to maintenance of local eosinophilia in schistosomal hepatic granulomas, and can function as immunoregulatory cells, besides their role in production of fibrosis. Electronic supplementary material: The online version of this article (doi:10.1186/s13071-015-1197-3) contains supplementary material, which is available to authorized users. �Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice (epmc).pdf DeepDyve Pubget Overpricing