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http://scihub22266oqcxt.onion/ C4646659!4646659!7150597     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\7150597.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biochim+Biophys+Acta 1982 ; 693 (1): 93-8 Nephropedia Template TP {{tp|p=7150597|t=1982. FUSION OF PHOSPHOLIPID VESICLES ARRESTED BY QUICK-FREEZING THE QUESTION OF LIPIDIC PARTICLES AS INTERMEDIATES IN MEMBRANE FUSION |pdf=|usr=}}{{7150597}} gab.com Text FUSION OF PHOSPHOLIPID VESICLES ARRESTED BY QUICK-FREEZING THE QUESTION OF LIPIDIC PARTICLES AS INTERMEDIATES IN MEMBRANE FUSION JO: Biochim Biophys Acta http://www.kidney.de/pget.php?&tw=1&pmid=7150597 #FOAvip We have examined the early events in Cai2+-induced fusion of large (0.2 ?m diameter) unilamellar cardiolipin/phosphatidylcholine and phosphatidylserine/phosphatidylethanolamine vesicles by quick-freezing freeze-fracture electron microscopy, eliminating the necessity of using glycerol as a cryoprotectant. Freeze-fracture replicas of vesicle suspensions frozen after 1-2 s of stimulation revealed that the majority of vesicles had already undergone membrane fusion, as evidenced by dumbbeII-shaped structures and large vesicles. In the absence of glycerol, lipidic particles or the hexagonal H 11phase, which have been proposed to be intermediate structures in membrane fusion, were not observed at the sites of fusion. Lipidic particles were evident in less than 5% of the cardiolipin/phosphatidylcholine vesicles after long-term incubation with Ca2+, and the addition of glycerol produced more vesicles displaying the particles. We have also shown that rapid fusion occurred within seconds of Ca2+ addition by the time-course of fluorescence emission produced by the intermixing of aqueous contents of two separate vesicle populations. These studies therefore have produced no evidence that lipidic particles are necessary intermediates for membrane fusion. On the contrary, they indicate that lipidic particles are structures obtained at equilibrium long after fusion has occurred and they become particularly prevalent in the presence of glycerol. Twit Text FOAVip FUSION OF PHOSPHOLIPID VESICLES ARRESTED BY QUICK-FREEZING THE QUESTION OF LIPIDIC PARTICLES AS INTERMEDIATES IN MEMBRANE FUSION ????Biochim Biophys Acta http://www.kidney.de/pget.php?&tw=1&pmid=7150597 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=7150597 #FOAvip English Wikipedia <ref>{{Cite pmid|7150597}}</ref> FUSION OF PHOSPHOLIPID VESICLES ARRESTED BY QUICK-FREEZING THE QUESTION OF LIPIDIC PARTICLES AS INTERMEDIATES IN MEMBRANE FUSION #MMPMID7150597 BEARER E; DÜZGÜNE? N; FRIEND D; PAPAHADJOPOULOS D Biochim Biophys Acta 1982[Dec]; 693 (1): 93-8 PMID7150597show ga We have examined the early events in Cai2+-induced fusion of large (0.2 ?m diameter) unilamellar cardiolipin/phosphatidylcholine and phosphatidylserine/phosphatidylethanolamine vesicles by quick-freezing freeze-fracture electron microscopy, eliminating the necessity of using glycerol as a cryoprotectant. Freeze-fracture replicas of vesicle suspensions frozen after 1-2 s of stimulation revealed that the majority of vesicles had already undergone membrane fusion, as evidenced by dumbbeII-shaped structures and large vesicles. In the absence of glycerol, lipidic particles or the hexagonal H 11phase, which have been proposed to be intermediate structures in membrane fusion, were not observed at the sites of fusion. Lipidic particles were evident in less than 5% of the cardiolipin/phosphatidylcholine vesicles after long-term incubation with Ca2+, and the addition of glycerol produced more vesicles displaying the particles. We have also shown that rapid fusion occurred within seconds of Ca2+ addition by the time-course of fluorescence emission produced by the intermixing of aqueous contents of two separate vesicle populations. These studies therefore have produced no evidence that lipidic particles are necessary intermediates for membrane fusion. On the contrary, they indicate that lipidic particles are structures obtained at equilibrium long after fusion has occurred and they become particularly prevalent in the presence of glycerol. äFUSION OF PHOSPHOLIPID VESICLES ARRESTED BY QUICK-FREEZING THE QUESTIO(epmc).pdf DeepDyve Pubget Overpricing