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10.1074/jbc.M115.710533 http://scihub22266oqcxt.onion/10.1074/jbc.M115.710533 C4861475!4861475!26896002     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26896002&cmd=llinks): Failed to open stream: HTTP request failed! 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Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 ?-Aggregated Structures in Influenza A Virus-infected Cells* |pdf=|usr=}}{{26896002}} gab.com Text Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 -Aggregated Structures in Influenza A Virus-infected Cells* JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26896002 #FOAvip PB1-F2 is a virulence factor of influenza A virus (IAV) whose functions remain misunderstood. The different roles of PB1-F2 may be linked to its structural polymorphism and to its propensity to assemble into oligomers and amyloid fibers in the vicinity of the membrane of IAV-infected cells. Here, we monitored the impact of PB1-F2 on the biochemical composition and protein structures of human epithelial pulmonary cells (A549) and monocytic cells (U937) upon IAV infection using synchrotron Fourier-transform infrared (FTIR) and deep UV (DUV) microscopies at the single-cell level. Cells were infected with a wild-type IAV and its PB1-F2 knock-out mutant for analyses at different times post-infection. IR spectra were recorded in each condition and processed to evaluate the change in the component band of the spectra corresponding to the amide I (secondary structure) and the CH stretching region (membrane). The IR spectra analysis revealed that expression of PB1-F2 in U937 cells, but not in A549 cells, results in the presence of a specific ?-aggregate signature. Furthermore, the lipid membrane composition of U937 cells expressing PB1-F2 was also altered in a cell type-dependent manner. Using DUV microscopy and taking advantage of the high content of tryptophan residues in the sequence of PB1-F2 (5/90 aa), we showed that the increase of the autofluorescent signal recorded in monocytic cells could be correlated with the IR detection of ?-aggregates. Altogether, our results constitute an important step forward in the understanding of the cell type-dependent function of PB1-F2. Twit Text FOAVip Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 -Aggregated Structures in Influenza A Virus-infected Cells* ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26896002 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26896002 #FOAvip English Wikipedia <ref>{{Cite pmid|26896002}}</ref> Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 ?-Aggregated Structures in Influenza A Virus-infected Cells* #MMPMID26896002 Chevalier C; Le Goffic R; Jamme F; Leymarie O; Réfrégiers M; Delmas B J Biol Chem 2016[Apr]; 291 (17): 9060-72 PMID26896002show ga PB1-F2 is a virulence factor of influenza A virus (IAV) whose functions remain misunderstood. The different roles of PB1-F2 may be linked to its structural polymorphism and to its propensity to assemble into oligomers and amyloid fibers in the vicinity of the membrane of IAV-infected cells. Here, we monitored the impact of PB1-F2 on the biochemical composition and protein structures of human epithelial pulmonary cells (A549) and monocytic cells (U937) upon IAV infection using synchrotron Fourier-transform infrared (FTIR) and deep UV (DUV) microscopies at the single-cell level. Cells were infected with a wild-type IAV and its PB1-F2 knock-out mutant for analyses at different times post-infection. IR spectra were recorded in each condition and processed to evaluate the change in the component band of the spectra corresponding to the amide I (secondary structure) and the CH stretching region (membrane). The IR spectra analysis revealed that expression of PB1-F2 in U937 cells, but not in A549 cells, results in the presence of a specific ?-aggregate signature. Furthermore, the lipid membrane composition of U937 cells expressing PB1-F2 was also altered in a cell type-dependent manner. Using DUV microscopy and taking advantage of the high content of tryptophan residues in the sequence of PB1-F2 (5/90 aa), we showed that the increase of the autofluorescent signal recorded in monocytic cells could be correlated with the IR detection of ?-aggregates. Altogether, our results constitute an important step forward in the understanding of the cell type-dependent function of PB1-F2. äSynchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study o(epmc).pdf DeepDyve Pubget Overpricing