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10.5812/jjm.28489 http://scihub22266oqcxt.onion/10.5812/jjm.28489 C4870841!4870841!27217919     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27217919.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Jundishapur+J+Microbiol 2016 ; 9 (3): ä Nephropedia Template TP {{tp|p=27217919|t=2016. In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein |pdf=|usr=}}{{27217919}} gab.com Text In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein JO: Jundishapur J Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27217919 #FOAvip Background:: Bacterial infection by antibiotic-resistant Staphylococcus aureus strains is a worldwide concern and the development of novel antistaphylococcal agents is acutely needed. Lysostaphin, an example of such novel agents, is a bacteriocin secreted by S. simulans to kill S. aureus through proteolysis of the Staphylococcus cell wall. Objectives:: The aim of this study was to evaluate the in vitro and in vivo antistaphylococcal activity of recombinant lysostaphin. Materials and Methods:: The in vitro study of the recombinant lysostaphin activity against S. aureus was determined by turbidimetric assay. For in vivo investigation, two groups of rats were inoculated with 1.4 × 109 CFU S. aureus. Five days after the nasal instillation of S. aureus, treatment in one of the groups was performed with a single dose (200 ?g/dose) of recombinant lysostaphin formulated in Eucerin-based cream. Results:: Recombinant lysostaphin at 100 ?g/mL concentration showed a significant decrease of the optical density compared to the control samples. The in vivo study demonstrated that a single dose (200 ?g/dose) of recombinant lysostaphin cream significantly reduced nasal colonization in all the treated animals compared to the untreated ones. Conclusions:: These results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal S. aureus in rats. It can be recommended for human clinical trial studies. Twit Text FOAVip In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein ????Jundishapur J Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27217919 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27217919 #FOAvip English Wikipedia <ref>{{Cite pmid|27217919}}</ref> In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein #MMPMID27217919 Abtahi H; Farhangnia L; Ghaznavi-Rad E Jundishapur J Microbiol 2016[Mar]; 9 (3): ä PMID27217919show ga Background:: Bacterial infection by antibiotic-resistant Staphylococcus aureus strains is a worldwide concern and the development of novel antistaphylococcal agents is acutely needed. Lysostaphin, an example of such novel agents, is a bacteriocin secreted by S. simulans to kill S. aureus through proteolysis of the Staphylococcus cell wall. Objectives:: The aim of this study was to evaluate the in vitro and in vivo antistaphylococcal activity of recombinant lysostaphin. Materials and Methods:: The in vitro study of the recombinant lysostaphin activity against S. aureus was determined by turbidimetric assay. For in vivo investigation, two groups of rats were inoculated with 1.4 × 109 CFU S. aureus. Five days after the nasal instillation of S. aureus, treatment in one of the groups was performed with a single dose (200 ?g/dose) of recombinant lysostaphin formulated in Eucerin-based cream. Results:: Recombinant lysostaphin at 100 ?g/mL concentration showed a significant decrease of the optical density compared to the control samples. The in vivo study demonstrated that a single dose (200 ?g/dose) of recombinant lysostaphin cream significantly reduced nasal colonization in all the treated animals compared to the untreated ones. Conclusions:: These results demonstrated that the recombinant lysostaphin produced in this study was able to kill nasal S. aureus in rats. It can be recommended for human clinical trial studies. äIn Vitro and in Vivo Antistaphylococcal Activity Determination of the (epmc).pdf DeepDyve Pubget Overpricing