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10.1093/abbs/gmw003 http://scihub22266oqcxt.onion/10.1093/abbs/gmw003 C4888355!4888355!26851484     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26851484.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Acta+Biochim+Biophys+Sin+(Shanghai) 2016 ; 48 (5): 468-73 Nephropedia Template TP {{tp|p=26851484|t=2016. Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis |pdf=|usr=}}{{26851484}} gab.com Text Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis JO: Acta Biochim Biophys Sin (Shanghai) http://www.kidney.de/pget.php?&tw=1&pmid=26851484 #FOAvip Major urinary proteins (MUPs) are the most abundant protein species in mouse urine, accounting for more than 90% of total protein content. Twenty-one Mup genes and 21 pseudogenes are clustered in a region of around 2 megabase pairs (Mbp) on chromosome 4. A Mup-knockout mouse model would greatly facilitate researches in the field of proteomic analysis of mouse urine. Here, we report the successful knockout of the Mup gene cluster of 2.2 Mbp using the CRISPR/Cas9 system. Homozygous Mup-knockout mice survived to adulthood and exhibited no obvious defects. The patterns of the proteomes of non-MUP urinary proteins in homozygous Mup-knockout mice were similar to those of wild-type mice judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The sensitivity of enzyme-linked immunosorbent assay to detect non-MUP urinary protein was significantly enhanced in Mup-knockout mice. In short, we have developed a Mup-knockout mouse model. This mouse model will be useful for the research of urinary biomarker testing that may have relevance for humans. Twit Text FOAVip Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis ????Acta Biochim Biophys Sin (Shanghai) http://www.kidney.de/pget.php?&tw=1&pmid=26851484 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26851484 #FOAvip English Wikipedia <ref>{{Cite pmid|26851484}}</ref> Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis #MMPMID26851484 Yang H; Zhang W; Lu S; Lu G; Zhang H; Zhuang Y; Wang Y; Dong M; Zhang Y; Zhou X; Wang P; Yu L; Wang F; Chen L Acta Biochim Biophys Sin (Shanghai) 2016[May]; 48 (5): 468-73 PMID26851484show ga Major urinary proteins (MUPs) are the most abundant protein species in mouse urine, accounting for more than 90% of total protein content. Twenty-one Mup genes and 21 pseudogenes are clustered in a region of around 2 megabase pairs (Mbp) on chromosome 4. A Mup-knockout mouse model would greatly facilitate researches in the field of proteomic analysis of mouse urine. Here, we report the successful knockout of the Mup gene cluster of 2.2 Mbp using the CRISPR/Cas9 system. Homozygous Mup-knockout mice survived to adulthood and exhibited no obvious defects. The patterns of the proteomes of non-MUP urinary proteins in homozygous Mup-knockout mice were similar to those of wild-type mice judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The sensitivity of enzyme-linked immunosorbent assay to detect non-MUP urinary protein was significantly enhanced in Mup-knockout mice. In short, we have developed a Mup-knockout mouse model. This mouse model will be useful for the research of urinary biomarker testing that may have relevance for humans. äMup-knockout mice generated through CRISPR/Cas9-mediated deletion for (epmc).pdf DeepDyve Pubget Overpricing