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10.1042/BCJ20160128 http://scihub22266oqcxt.onion/10.1042/BCJ20160128 C4928857!4928857!26987815     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26987815&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26987815.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biochem+J 2016 ; 473 (10): 1455-69 Nephropedia Template TP {{tp|p=26987815|t=2016. ?B-crystallin is essential for the TGF-?2-mediated epithelial to mesenchymal transition of lens epithelial cells |pdf=|usr=}}{{26987815}} gab.com Text B-crystallin is essential for the TGF- 2-mediated epithelial to mesenchymal transition of lens epithelial cells JO: Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=26987815 #FOAvip Transforming growth factor (TGF)-?2-mediated pathways play a major role in the epithelial to mesenchymal transition (EMT) of lens epithelial cells (LECs) during secondary cataract formation, which is also known as posterior capsule opacification (PCO). Although ?B-crystallin is a major protein in LEC, its role in the EMT remains unknown. In a human LEC line (FHL124), TGF-?2 treatment resulted in changes in the EMT-associated proteins at the mRNA and protein levels. This was associated with nuclear localization of ?B-crystallin, phosphorylated Smad2 (pSmad2) (S245/250/255), pSmad3 (S423/425), Smad4 and Snail and the binding of ?B-crystallin to these transcription factors, all of which were reduced by the down-regulation of ?B-crystallin. Expression of the functionally defective R120G mutant of ?B-crystallin reduced TGF-?2-induced EMT in LECs of ?B-crystallin knockout (KO) mice. Treatment of bovine lens epithelial explants and mouse LEC with TGF-?2 resulted in changes in the EMT-associated proteins at the mRNA and protein levels. This was accompanied by increase in phosphorylation of p44/42 mitogen-activated protein kinases (MAPK) (T202/Y204), p38 MAPK (T180/Y182), protein kinase B (Akt) (S473) and Smad2 when compared with untreated cells. These changes were significantly reduced in ?B-crystallin depleted or knocked out LEC. The removal of the fibre cell mass from the lens of wild-type (WT) mice resulted in the up-regulation of EMT-associated genes in the capsule-adherent epithelial cells, which was reduced in the ?B-crystallin KO mice. Together, our data show that ?B-crystallin plays a central role in the TGF-?2-induced EMT of LEC. ?B-Crystallin could be targeted to prevent PCO and pathological fibrosis in other tissues. Twit Text FOAVip B-crystallin is essential for the TGF- 2-mediated epithelial to mesenchymal transition of lens epithelial cells ????Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=26987815 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26987815 #FOAvip English Wikipedia <ref>{{Cite pmid|26987815}}</ref> ?B-crystallin is essential for the TGF-?2-mediated epithelial to mesenchymal transition of lens epithelial cells #MMPMID26987815 Nahomi RB; Pantcheva MB; Nagaraj RH Biochem J 2016[May]; 473 (10): 1455-69 PMID26987815show ga Transforming growth factor (TGF)-?2-mediated pathways play a major role in the epithelial to mesenchymal transition (EMT) of lens epithelial cells (LECs) during secondary cataract formation, which is also known as posterior capsule opacification (PCO). Although ?B-crystallin is a major protein in LEC, its role in the EMT remains unknown. In a human LEC line (FHL124), TGF-?2 treatment resulted in changes in the EMT-associated proteins at the mRNA and protein levels. This was associated with nuclear localization of ?B-crystallin, phosphorylated Smad2 (pSmad2) (S245/250/255), pSmad3 (S423/425), Smad4 and Snail and the binding of ?B-crystallin to these transcription factors, all of which were reduced by the down-regulation of ?B-crystallin. Expression of the functionally defective R120G mutant of ?B-crystallin reduced TGF-?2-induced EMT in LECs of ?B-crystallin knockout (KO) mice. Treatment of bovine lens epithelial explants and mouse LEC with TGF-?2 resulted in changes in the EMT-associated proteins at the mRNA and protein levels. This was accompanied by increase in phosphorylation of p44/42 mitogen-activated protein kinases (MAPK) (T202/Y204), p38 MAPK (T180/Y182), protein kinase B (Akt) (S473) and Smad2 when compared with untreated cells. These changes were significantly reduced in ?B-crystallin depleted or knocked out LEC. The removal of the fibre cell mass from the lens of wild-type (WT) mice resulted in the up-regulation of EMT-associated genes in the capsule-adherent epithelial cells, which was reduced in the ?B-crystallin KO mice. Together, our data show that ?B-crystallin plays a central role in the TGF-?2-induced EMT of LEC. ?B-Crystallin could be targeted to prevent PCO and pathological fibrosis in other tissues. ä?B-crystallin is essential for the TGF-?2-mediated epithelial to mesen(epmc).pdf DeepDyve Pubget Overpricing