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10.4103/1742-6413.200936 http://scihub22266oqcxt.onion/10.4103/1742-6413.200936 C5348988!5348988!28331530     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28331530&cmd=llinks): Failed to open stream: HTTP request failed! 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KRAS detection on archival cytological smears by the novel fully automated polymerase chain reaction-based Idylla mutation test |pdf=|usr=}}{{28331530}} gab.com Text KRAS detection on archival cytological smears by the novel fully automated polymerase chain reaction-based Idylla mutation test JO: Cytojournal http://www.kidney.de/pget.php?&tw=1&pmid=28331530 #FOAvip Background:: Molecular techniques are relevant to modern cytopathology, but their implementation is difficult without molecular expertise and infrastructure. The assessment of KRAS mutational status on cytological preparations may be useful either to refine uncertain diagnoses on pancreatic aspirates or to yield predictive information to plan targeted treatment of metastatic colorectal cancer (mCRC). The novel test Idylla? enables fully automated KRAS genotyping in approximately 2 h, even in less experienced hands. Materials and Methods:: This study aims to validate this methodology to detect KRAS mutations on archival cytological preparations of pancreatic cancer (n = 9) and mCRC (n = 9) by comparing the Idylla? performance to that of standard real-time polymerase chain reaction. Results:: The same 11 mutations (n = 4: p.G12D; n = 2: p.G12V; n = 2: p.A59E/G/T; n = 1: p.G12R; n = 1: p.G13D; n = 1: p.Q61H) were detected by both techniques. Conclusion:: Even in less experienced laboratories, a cytopathologist may easily integrate morphological diagnostic report with accurate KRAS mutation detection, which is relevant for diagnostic and treatment decisions. Twit Text FOAVip KRAS detection on archival cytological smears by the novel fully automated polymerase chain reaction-based Idylla mutation test ????Cytojournal http://www.kidney.de/pget.php?&tw=1&pmid=28331530 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28331530 #FOAvip English Wikipedia <ref>{{Cite pmid|28331530}}</ref> KRAS detection on archival cytological smears by the novel fully automated polymerase chain reaction-based Idylla mutation test #MMPMID28331530 De Luca C; Vigliar E; d?Anna M; Pisapia P; Bellevicine C; Malapelle U; Troncone G Cytojournal 2017[]; 14 (ä): ä PMID28331530show ga Background:: Molecular techniques are relevant to modern cytopathology, but their implementation is difficult without molecular expertise and infrastructure. The assessment of KRAS mutational status on cytological preparations may be useful either to refine uncertain diagnoses on pancreatic aspirates or to yield predictive information to plan targeted treatment of metastatic colorectal cancer (mCRC). The novel test Idylla? enables fully automated KRAS genotyping in approximately 2 h, even in less experienced hands. Materials and Methods:: This study aims to validate this methodology to detect KRAS mutations on archival cytological preparations of pancreatic cancer (n = 9) and mCRC (n = 9) by comparing the Idylla? performance to that of standard real-time polymerase chain reaction. Results:: The same 11 mutations (n = 4: p.G12D; n = 2: p.G12V; n = 2: p.A59E/G/T; n = 1: p.G12R; n = 1: p.G13D; n = 1: p.Q61H) were detected by both techniques. Conclusion:: Even in less experienced laboratories, a cytopathologist may easily integrate morphological diagnostic report with accurate KRAS mutation detection, which is relevant for diagnostic and treatment decisions. äKRAS detection on archival cytological smears by the novel fully autom(epmc).pdf DeepDyve Pubget Overpricing