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10.1371/journal.pone.0173891 http://scihub22266oqcxt.onion/10.1371/journal.pone.0173891 C5362207!5362207!28329012     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28329012&cmd=llinks): Failed to open stream: HTTP request failed! 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Tracking the stochastic fate of cells of the renin lineage after podocyte depletion using multicolor reporters and intravital imaging |pdf=|usr=}}{{28329012}} gab.com Text Tracking the stochastic fate of cells of the renin lineage after podocyte depletion using multicolor reporters and intravital imaging JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28329012 #FOAvip Podocyte depletion plays a major role in focal segmental glomerular sclerosis (FSGS). Because cells of the renin lineage (CoRL) serve as adult podocyte and parietal epithelial cell (PEC) progenitor candidates, we generated Ren1cCre/R26R-ConfettiTG/WT and Ren1dCre/R26R-ConfettiTG/WT mice to determine CoRL clonality during podocyte replacement. Four CoRL reporters (GFP, YFP, RFP, CFP) were restricted to cells in the juxtaglomerular compartment (JGC) at baseline. Following abrupt podocyte depletion in experimental FSGS, all four CoRL reporters were detected in a subset of glomeruli at day 28, where they co-expressed de novo four podocyte proteins (podocin, nephrin, WT-1 and p57) and two glomerular parietal epithelial cell (PEC) proteins (claudin-1, PAX8). To monitor the precise migration of a subset of CoRL over a 2w period following podocyte depletion, intravital multiphoton microscopy was used. Our findings demonstrate direct visual support for the migration of single CoRL from the JGC to the parietal Bowman?s capsule, early proximal tubule, mesangium and glomerular tuft. In summary, these results suggest that following podocyte depletion, multi-clonal CoRL migrate to the glomerulus and replace podocyte and PECs in experimental FSGS. Twit Text FOAVip Tracking the stochastic fate of cells of the renin lineage after podocyte depletion using multicolor reporters and intravital imaging ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28329012 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28329012 #FOAvip English Wikipedia <ref>{{Cite pmid|28329012}}</ref> Tracking the stochastic fate of cells of the renin lineage after podocyte depletion using multicolor reporters and intravital imaging #MMPMID28329012 Kaverina NV; Kadoya H; Eng DG; Rusiniak ME; Sequeira-Lopez MLS; Gomez RA; Pippin JW; Gross KW; Peti-Peterdi J; Shankland SJ PLoS One 2017[]; 12 (3): ä PMID28329012show ga Podocyte depletion plays a major role in focal segmental glomerular sclerosis (FSGS). Because cells of the renin lineage (CoRL) serve as adult podocyte and parietal epithelial cell (PEC) progenitor candidates, we generated Ren1cCre/R26R-ConfettiTG/WT and Ren1dCre/R26R-ConfettiTG/WT mice to determine CoRL clonality during podocyte replacement. Four CoRL reporters (GFP, YFP, RFP, CFP) were restricted to cells in the juxtaglomerular compartment (JGC) at baseline. Following abrupt podocyte depletion in experimental FSGS, all four CoRL reporters were detected in a subset of glomeruli at day 28, where they co-expressed de novo four podocyte proteins (podocin, nephrin, WT-1 and p57) and two glomerular parietal epithelial cell (PEC) proteins (claudin-1, PAX8). To monitor the precise migration of a subset of CoRL over a 2w period following podocyte depletion, intravital multiphoton microscopy was used. Our findings demonstrate direct visual support for the migration of single CoRL from the JGC to the parietal Bowman?s capsule, early proximal tubule, mesangium and glomerular tuft. In summary, these results suggest that following podocyte depletion, multi-clonal CoRL migrate to the glomerulus and replace podocyte and PECs in experimental FSGS. äTracking the stochastic fate of cells of the renin lineage after podoc(epmc).pdf DeepDyve Pubget Overpricing