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TISSUE MYELOID PROGENITORS DIFFERENTIATE INTO PERICYTES THROUGH TGF-? SIGNALING IN DEVELOPING SKIN VASCULATURE #MMPMID28329690
Yamazaki T; Nalbandian A; Uchida Y; Li W; Arnold TD; Kubota Y; Yamamoto S; Ema M; Mukouyama Ys
Cell Rep 2017[Mar]; 18 (12): 2991-3004 PMID28329690show ga
Mural cells (pericytes and vascular smooth muscle cells) are essential for the regulation of vascular networks and maintenance of vascular integrity, but their origins are diverse in different tissues and not known in the organs that arise from the ectoderm, such as skin. Here we show that tissue-localized myeloid progenitors contribute to pericyte development in embryonic skin vasculature. A series of in vivo fate-mapping experiments indicates that tissue myeloid progenitors differentiate into pericytes. Furthermore, depletion of tissue myeloid cells and their progenitors in PU.1 mutants results in defective pericyte development. FACS-isolated myeloid cells and their progenitors from embryonic skin differentiate into pericytes in culture. At the molecular level, transforming growth factor-? (TGF-?) induces pericyte differentiation in culture. Furthermore, type2 TGF-? receptor (Tgfbr2) mutants exhibit deficient pericyte development in skin vasculature. Combined, these data suggest that pericytes differentiate from tissue myeloid progenitors in the skin vasculature through TGF-? signaling.