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10.1364/BOE.8.003470

http://scihub22266oqcxt.onion/10.1364/BOE.8.003470
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C5508843!5508843!28717582
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suck abstract from ncbi


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pmid28717582      Biomed+Opt+Express 2017 ; 8 (7): 3470-81
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  • In vivo multiphoton imaging of a diverse array of fluorophores to investigate deep neurovascular structure #MMPMID28717582
  • Miller DR; Hassan AM; Jarrett JW; Medina FA; Perillo EP; Hagan K; Shams Kazmi SM; Clark TA; Sullender CT; Jones TA; Zemelman BV; Dunn AK
  • Biomed Opt Express 2017[Jul]; 8 (7): 3470-81 PMID28717582show ga
  • We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between ?=1,100 and 1,400 nm. By combining the high repetition rate (511 kHz) and high pulse energy (400 nJ) of our amplifier laser system, we demonstrate imaging of vasculature labeled with Texas Red and Indocyanine Green, and neurons expressing tdTomato and yellow fluorescent protein. We measure the blood flow speed of a single capillary at a depth of 1.2 mm, and image vasculature to a depth of 1.53 mm with fine axial steps (5 ?m) and reasonable acquisition times. The high image quality enabled analysis of vascular morphology at depths to 1.45 mm.
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