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10.1038/s41598-017-11152-w http://scihub22266oqcxt.onion/10.1038/s41598-017-11152-w C5587571!5587571!28878312     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28878312&cmd=llinks): Failed to open stream: HTTP request failed! 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SERS-based Immunoassay in a Microfluidic System for the Multiplexed Recognition of Interleukins from Blood Plasma: Towards Picogram Detection |pdf=|usr=}}{{28878312}} gab.com Text SERS-based Immunoassay in a Microfluidic System for the Multiplexed Recognition of Interleukins from Blood Plasma: Towards Picogram Detection JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28878312 #FOAvip SERS-active nanostructures incorporated into a microfluidic device have been developed for rapid and multiplex monitoring of selected Type 1 cytokine (interleukins: IL-6, IL-8, IL-18) levels in blood plasma. Multiple analyses have been performed by using nanoparticles, each coated with different Raman reporter molecules: 5,5?-dithio-bis(2-nitro-benzoic acid) (DTNB), fuchsin (FC), and p-mercatpobenzoic acid (p-MBA) and with specific antibodies. The multivariate statistical method, principal component analysis (PCA), was applied for segregation of three different antigen-antibody complexes encoded by three Raman reporters (FC, p-MBA, and DTNB) during simultaneous multiplexed detection approach. To the best of our knowledge, we have also presented, for the first time, a possibility for multiplexed quantification of three interleukins: IL-6, IL-8, and IL-18 in blood plasma samples using SERS technique. Our method improves the detection limit in comparison to standard ELISA methods. The low detection limits were estimated to be 2.3?pg�ml?1, 6.5?pg�ml?1, and 4.2?pg�ml?1 in a parallel approach, and 3.8?pg�ml?1, 7.5?pg�ml?1, and 5.2?pg�ml?1 in a simultaneous multiplexed method for IL-6, IL-8, and IL-18, respectively. This demonstrated the sensitivity and reproducibility desirable for analytical examinations. Twit Text FOAVip SERS-based Immunoassay in a Microfluidic System for the Multiplexed Recognition of Interleukins from Blood Plasma: Towards Picogram Detection ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28878312 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28878312 #FOAvip English Wikipedia <ref>{{Cite pmid|28878312}}</ref> SERS-based Immunoassay in a Microfluidic System for the Multiplexed Recognition of Interleukins from Blood Plasma: Towards Picogram Detection #MMPMID28878312 Kami?ska A; Winkler K; Kowalska A; Witkowska E; Szymborski T; Janeczek A; Waluk J Sci Rep 2017[]; 7 (�): � PMID28878312show ga SERS-active nanostructures incorporated into a microfluidic device have been developed for rapid and multiplex monitoring of selected Type 1 cytokine (interleukins: IL-6, IL-8, IL-18) levels in blood plasma. Multiple analyses have been performed by using nanoparticles, each coated with different Raman reporter molecules: 5,5?-dithio-bis(2-nitro-benzoic acid) (DTNB), fuchsin (FC), and p-mercatpobenzoic acid (p-MBA) and with specific antibodies. The multivariate statistical method, principal component analysis (PCA), was applied for segregation of three different antigen-antibody complexes encoded by three Raman reporters (FC, p-MBA, and DTNB) during simultaneous multiplexed detection approach. To the best of our knowledge, we have also presented, for the first time, a possibility for multiplexed quantification of three interleukins: IL-6, IL-8, and IL-18 in blood plasma samples using SERS technique. Our method improves the detection limit in comparison to standard ELISA methods. The low detection limits were estimated to be 2.3?pg�ml?1, 6.5?pg�ml?1, and 4.2?pg�ml?1 in a parallel approach, and 3.8?pg�ml?1, 7.5?pg�ml?1, and 5.2?pg�ml?1 in a simultaneous multiplexed method for IL-6, IL-8, and IL-18, respectively. This demonstrated the sensitivity and reproducibility desirable for analytical examinations. �SERS-based Immunoassay in a Microfluidic System for the Multiplexed Re(epmc).pdf DeepDyve Pubget Overpricing