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10.1039/c6nr06417e http://scihub22266oqcxt.onion/10.1039/c6nr06417e C5695240!5695240!27845796     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27845796&cmd=llinks): Failed to open stream: HTTP request failed! 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Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens? |pdf=|usr=}}{{27845796}} gab.com Text Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens JO: Nanoscale http://www.kidney.de/pget.php?&tw=1&pmid=27845796 #FOAvip Rapid and sensitive point-of-care diagnostics are of paramount importance for early detection of infectious diseases and timely initiation of treatment. Here, we present cellulose paper and flexible plastic chips with printed graphene-modified silver electrodes as universal point-of-care diagnostic tools for the rapid and sensitive detection of microbial pathogens or nucleic acids through utilizing electrical sensing modality and loop-mediated isothermal amplification (LAMP). We evaluated the ability of the developed paper-based assay to detect (i) viruses on cellulose-based paper microchips without implementing amplification in samples with viral loads between 106 and 108 copies per ml, and (ii) amplified HIV-1 nucleic acids in samples with viral loads between 10 fg �l?1 and 108 fg �l?1. The target HIV-1 nucleic acid was amplified using the RT-LAMP technique and detected through the electrical sensing of LAMP amplicons for a broad range of RNA concentrations between 10 fg �l?1 and 108 fg �l?1 after 40 min of amplification time. Our assay may be used for antiretroviral therapy monitoring where it meets the sensitivity requirement of the World Health Organization guidelines. Such a paper microchip assay without the amplification step may also be considered as a simple and inexpensive approach for acute HIV detection where maximum viral replication occurs. Twit Text FOAVip Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens ????Nanoscale http://www.kidney.de/pget.php?&tw=1&pmid=27845796 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27845796 #FOAvip English Wikipedia <ref>{{Cite pmid|27845796}}</ref> Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens? #MMPMID27845796 Safavieh M; Kaul V; Khetani S; Singh A; Dhingra K; Kanakasabapathy MK; Draz MS; Memic A; Kuritzkes DR; Shafiee H Nanoscale 2017[Feb]; 9 (5): 1852-61 PMID27845796show ga Rapid and sensitive point-of-care diagnostics are of paramount importance for early detection of infectious diseases and timely initiation of treatment. Here, we present cellulose paper and flexible plastic chips with printed graphene-modified silver electrodes as universal point-of-care diagnostic tools for the rapid and sensitive detection of microbial pathogens or nucleic acids through utilizing electrical sensing modality and loop-mediated isothermal amplification (LAMP). We evaluated the ability of the developed paper-based assay to detect (i) viruses on cellulose-based paper microchips without implementing amplification in samples with viral loads between 106 and 108 copies per ml, and (ii) amplified HIV-1 nucleic acids in samples with viral loads between 10 fg �l?1 and 108 fg �l?1. The target HIV-1 nucleic acid was amplified using the RT-LAMP technique and detected through the electrical sensing of LAMP amplicons for a broad range of RNA concentrations between 10 fg �l?1 and 108 fg �l?1 after 40 min of amplification time. Our assay may be used for antiretroviral therapy monitoring where it meets the sensitivity requirement of the World Health Organization guidelines. Such a paper microchip assay without the amplification step may also be considered as a simple and inexpensive approach for acute HIV detection where maximum viral replication occurs. �Paper microchip with a graphene-modified silver nano-composite electro(epmc).pdf DeepDyve Pubget Overpricing