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10.1111/jcmm.13442 http://scihub22266oqcxt.onion/10.1111/jcmm.13442 C5824402!5824402!29349903     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29349903.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Mol+Med 2018 ; 22 (3): 1650-65 Nephropedia Template TP {{tp|p=29349903|t=2018. GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial?mesenchymal transition through the TGF??1/Smad2 signalling pathway |pdf=|usr=}}{{29349903}} gab.com Text GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial mesenchymal transition through the TGF 1/Smad2 signalling pathway JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=29349903 #FOAvip This study investigated the effects of Golgi membrane protein 73 (GP73) on the epithelial?mesenchymal transition (EMT) and on bladder cancer cell invasion and metastasis through the TGF??1/Smad2 signalling pathway. Paired bladder cancer and adjacent tissue samples (102) and normal bladder tissue samples (106) were obtained. Bladder cancer cell lines (T24, 5637, RT4, 253J and J82) were selected and assigned to blank, negative control (NC), TGF??, thrombospondin?1 (TSP?1), TGF??1+ TSP?1, GP73?siRNA?1, GP73?siRNA?2, GP73?siRNA?1+ TSP?1, GP73?siRNA?1+ pcDNA?GP73, WT1?siRNA and WT1?siRNA + GP73?siRNA?1 groups. Expressions of GP73, TGF??1, Smad2, p?Smad2, E?cadherin and vimentin were detected using RT?qPCR and Western blotting. Cell proliferation, migration and invasion were determined using MTT assay, scratch testing and Transwell assay, respectively. Compared with the blank and NC groups, levels of GP73, TGF??1, Smad2, p?Smad2, N?cadherin and vimentin decreased, and levels of WT1 and E?cadherin increased in the GP73?siRNA?1 and GP73?siRNA?2 groups, while the opposite results were observed in the WT1 siRNA, TGF??, TSP?1 and TGF?? + TSP?1 groups. Cell proliferation, migration and invasion notably decreased in the GP73?siRNA?1 and GP73?siRNA?2 groups in comparison with the blank and NC groups, while in the WT1 siRNA, TGF??, TSP?1 and TGF?? + TSP?1 groups, cell migration, invasion and proliferation showed the reduction after the EMT. These results suggest that GP73 promotes bladder cancer invasion and metastasis by inducing the EMT through down?regulating WT1 levels and activating the TGF??1/Smad2 signalling pathway. Twit Text FOAVip GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial mesenchymal transition through the TGF 1/Smad2 signalling pathway ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=29349903 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29349903 #FOAvip English Wikipedia <ref>{{Cite pmid|29349903}}</ref> GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial?mesenchymal transition through the TGF??1/Smad2 signalling pathway #MMPMID29349903 Yang H; Liu G; Liu B; Liu T J Cell Mol Med 2018[Mar]; 22 (3): 1650-65 PMID29349903show ga This study investigated the effects of Golgi membrane protein 73 (GP73) on the epithelial?mesenchymal transition (EMT) and on bladder cancer cell invasion and metastasis through the TGF??1/Smad2 signalling pathway. Paired bladder cancer and adjacent tissue samples (102) and normal bladder tissue samples (106) were obtained. Bladder cancer cell lines (T24, 5637, RT4, 253J and J82) were selected and assigned to blank, negative control (NC), TGF??, thrombospondin?1 (TSP?1), TGF??1+ TSP?1, GP73?siRNA?1, GP73?siRNA?2, GP73?siRNA?1+ TSP?1, GP73?siRNA?1+ pcDNA?GP73, WT1?siRNA and WT1?siRNA + GP73?siRNA?1 groups. Expressions of GP73, TGF??1, Smad2, p?Smad2, E?cadherin and vimentin were detected using RT?qPCR and Western blotting. Cell proliferation, migration and invasion were determined using MTT assay, scratch testing and Transwell assay, respectively. Compared with the blank and NC groups, levels of GP73, TGF??1, Smad2, p?Smad2, N?cadherin and vimentin decreased, and levels of WT1 and E?cadherin increased in the GP73?siRNA?1 and GP73?siRNA?2 groups, while the opposite results were observed in the WT1 siRNA, TGF??, TSP?1 and TGF?? + TSP?1 groups. Cell proliferation, migration and invasion notably decreased in the GP73?siRNA?1 and GP73?siRNA?2 groups in comparison with the blank and NC groups, while in the WT1 siRNA, TGF??, TSP?1 and TGF?? + TSP?1 groups, cell migration, invasion and proliferation showed the reduction after the EMT. These results suggest that GP73 promotes bladder cancer invasion and metastasis by inducing the EMT through down?regulating WT1 levels and activating the TGF??1/Smad2 signalling pathway. äGP73 promotes invasion and metastasis of bladder cancer by regulating (epmc).pdf DeepDyve Pubget Overpricing