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10.1371/journal.pone.0195542 http://scihub22266oqcxt.onion/10.1371/journal.pone.0195542 C5896948!5896948!29649293     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29649293&cmd=llinks): Failed to open stream: HTTP request failed! 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Inducing cell death in vitro in cancer cells by targeted delivery of cytochrome c via a transferrin conjugate |pdf=|usr=}}{{29649293}} gab.com Text Inducing cell death in vitro in cancer cells by targeted delivery of cytochrome c via a transferrin conjugate JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=29649293 #FOAvip One of the major drawbacks of many of the currently used cancer drugs are off-target effects. Targeted delivery is one method to minimize such unwanted and detrimental events. To actively target lung cancer cells, we have developed a conjugate of the apoptosis inducing protein cytochrome c with transferrin because the transferrin receptor is overexpressed by many rapidly dividing cancer cells. Cytochrome c and transferrin were cross-linked with a redox sensitive disulfide bond for the intra-cellular release of the protein upon endocytosis by the transferrin receptor. Confocal results demonstrated the cellular uptake of the cytochrome c-transferrin conjugate by transferrin receptor overexpressing A549 lung cancer cells. Localization studies further validated that this conjugate escaped the endosome. Additionally, an in vitro assay showed that the conjugate could induce apoptosis by activating caspase-3. The neo-conjugate not only maintained an IC50 value similar to the well known drug cisplatin (50 ?M) in A549 cancer cells but also was nontoxic to the normal lung (MRC5) cells. Our neo-conjugate holds promise for future development to target cancers with enhanced transferrin receptor expression. Twit Text FOAVip Inducing cell death in vitro in cancer cells by targeted delivery of cytochrome c via a transferrin conjugate ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=29649293 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29649293 #FOAvip English Wikipedia <ref>{{Cite pmid|29649293}}</ref> Inducing cell death in vitro in cancer cells by targeted delivery of cytochrome c via a transferrin conjugate #MMPMID29649293 Saxena M; Delgado Y; Sharma RK; Sharma S; Guzmán SLPDL; Tinoco AD; Griebenow K PLoS One 2018[]; 13 (4): ä PMID29649293show ga One of the major drawbacks of many of the currently used cancer drugs are off-target effects. Targeted delivery is one method to minimize such unwanted and detrimental events. To actively target lung cancer cells, we have developed a conjugate of the apoptosis inducing protein cytochrome c with transferrin because the transferrin receptor is overexpressed by many rapidly dividing cancer cells. Cytochrome c and transferrin were cross-linked with a redox sensitive disulfide bond for the intra-cellular release of the protein upon endocytosis by the transferrin receptor. Confocal results demonstrated the cellular uptake of the cytochrome c-transferrin conjugate by transferrin receptor overexpressing A549 lung cancer cells. Localization studies further validated that this conjugate escaped the endosome. Additionally, an in vitro assay showed that the conjugate could induce apoptosis by activating caspase-3. The neo-conjugate not only maintained an IC50 value similar to the well known drug cisplatin (50 ?M) in A549 cancer cells but also was nontoxic to the normal lung (MRC5) cells. Our neo-conjugate holds promise for future development to target cancers with enhanced transferrin receptor expression. äInducing cell death in vitro in cancer cells by targeted delivery of c(epmc).pdf DeepDyve Pubget Overpricing