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10.1111/jcmm.13511 http://scihub22266oqcxt.onion/10.1111/jcmm.13511 C5908097!5908097!29478261     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29478261&cmd=llinks): Failed to open stream: HTTP request failed! 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Myocardial infarction stabilization by cell?based expression of controlled Vascular Endothelial Growth Factor levels |pdf=|usr=}}{{29478261}} gab.com Text Myocardial infarction stabilization by cell based expression of controlled Vascular Endothelial Growth Factor levels JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=29478261 #FOAvip Vascular Endothelial Growth Factor (VEGF) can induce normal or aberrant angiogenesis depending on the amount secreted in the microenvironment around each cell. Towards a possible clinical translation, we developed a Fluorescence Activated Cell Sorting (FACS)?based technique to rapidly purify transduced progenitors that homogeneously express a desired specific VEGF level from heterogeneous primary populations. Here, we sought to induce safe and functional angiogenesis in ischaemic myocardium by cell?based expression of controlled VEGF levels. Human adipose stromal cells (ASC) were transduced with retroviral vectors and FACS purified to generate two populations producing similar total VEGF doses, but with different distributions: one with cells homogeneously producing a specific VEGF level (SPEC), and one with cells heterogeneously producing widespread VEGF levels (ALL), but with an average similar to that of the SPEC population. A total of 70 nude rats underwent myocardial infarction by coronary artery ligation and 2�weeks later VEGF?expressing or control cells, or saline were injected at the infarction border. Four weeks later, ventricular ejection fraction was significantly worsened with all treatments except for SPEC cells. Further, only SPEC cells significantly increased the density of homogeneously normal and mature microvascular networks. This was accompanied by a positive remodelling effect, with significantly reduced fibrosis in the infarcted area. We conclude that controlled homogeneous VEGF delivery by FACS?purified transduced ASC is a promising strategy to achieve safe and functional angiogenesis in myocardial ischaemia. Twit Text FOAVip Myocardial infarction stabilization by cell based expression of controlled Vascular Endothelial Growth Factor levels ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=29478261 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29478261 #FOAvip English Wikipedia <ref>{{Cite pmid|29478261}}</ref> Myocardial infarction stabilization by cell?based expression of controlled Vascular Endothelial Growth Factor levels #MMPMID29478261 Melly L; Cerino G; Frobert A; Cook S; Giraud M; Carrel T; Tevaearai Stahel HT; Eckstein F; Rondelet B; Marsano A; Banfi A J Cell Mol Med 2018[May]; 22 (5): 2580-91 PMID29478261show ga Vascular Endothelial Growth Factor (VEGF) can induce normal or aberrant angiogenesis depending on the amount secreted in the microenvironment around each cell. Towards a possible clinical translation, we developed a Fluorescence Activated Cell Sorting (FACS)?based technique to rapidly purify transduced progenitors that homogeneously express a desired specific VEGF level from heterogeneous primary populations. Here, we sought to induce safe and functional angiogenesis in ischaemic myocardium by cell?based expression of controlled VEGF levels. Human adipose stromal cells (ASC) were transduced with retroviral vectors and FACS purified to generate two populations producing similar total VEGF doses, but with different distributions: one with cells homogeneously producing a specific VEGF level (SPEC), and one with cells heterogeneously producing widespread VEGF levels (ALL), but with an average similar to that of the SPEC population. A total of 70 nude rats underwent myocardial infarction by coronary artery ligation and 2�weeks later VEGF?expressing or control cells, or saline were injected at the infarction border. Four weeks later, ventricular ejection fraction was significantly worsened with all treatments except for SPEC cells. Further, only SPEC cells significantly increased the density of homogeneously normal and mature microvascular networks. This was accompanied by a positive remodelling effect, with significantly reduced fibrosis in the infarcted area. We conclude that controlled homogeneous VEGF delivery by FACS?purified transduced ASC is a promising strategy to achieve safe and functional angiogenesis in myocardial ischaemia. �Myocardial infarction stabilization by cell?based expression of contro(epmc).pdf DeepDyve Pubget Overpricing