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10.1007/978-1-4939-7902-8_11

http://scihub22266oqcxt.onion/10.1007/978-1-4939-7902-8_11
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C6020991!6020991!29892821
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suck abstract from ncbi


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pmid29892821      Methods+Mol+Biol 2018 ; 1801 (�): 111-22
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  • Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro #MMPMID29892821
  • Zhou J; Sinha RA; Lesmana R; Yau WWY; Yen PM
  • Methods Mol Biol 2018[]; 1801 (�): 111-22 PMID29892821show ga
  • Autophagy is an evolutionarily conserved intracellular catabolic process that is essential for cellular housekeeping and nutrient homeostasis. Recently, we provided evidence that thyroid hormone (TH) is a major inducer of autophagy in mammalian cells. Here, we describe a method for detecting TH-induced autophagic flux in hepatic, muscle, and brown adipocyte cells using lysosomal inhibitor bafilomycin A1 (BafA1) and conventional Western blot techniques.



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