
| 10.1038/sdata.2018.129
http://scihub22266oqcxt.onion/10.1038/sdata.2018.129
 C6049032!6049032!30015806
free
free
free
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Sci+Data 2018 ; 5 (�): � Nephropedia Template TP
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Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos #MMPMID30015806Pizzagalli DU; Farsakoglu Y; Palomino-Segura M; Palladino E; Sintes J; Marangoni F; Mempel TR; Koh WH; Murooka TT; Thelen F; Stein JV; Pozzi G; Thelen M; Krause R; Gonzalez SFSci Data 2018[]; 5 (�): � PMID30015806show ga
Recent advances in intravital video microscopy have allowed the visualization of leukocyte behavior in vivo, revealing unprecedented spatiotemporal dynamics of immune cell interaction. However, state-of-the-art software and methods for automatically measuring cell migration exhibit limitations in tracking the position of leukocytes over time. Challenges arise both from the complex migration patterns of these cells and from the experimental artifacts introduced during image acquisition. Additionally, the development of novel tracking tools is hampered by the lack of a sound ground truth for algorithm validation and benchmarking. Therefore, the objective of this work was to create a database, namely LTDB, with a significant number of manually tracked leukocytes. Broad experimental conditions, sites of imaging, types of immune cells and challenging case studies were included to foster the development of robust computer vision techniques for imaging-based immunological research. Lastly, LTDB represents a step towards the unravelling of biological mechanisms by video data mining in systems biology.�
  
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