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10.1002/bmc.4238 http://scihub22266oqcxt.onion/10.1002/bmc.4238 C6055858!6055858!29517154     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29517154&cmd=llinks): Failed to open stream: HTTP request failed! 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Quantification of cystine in human renal proximal tubule cells using liquid chromatography?tandem mass spectrometry |pdf=|usr=}}{{29517154}} gab.com Text Quantification of cystine in human renal proximal tubule cells using liquid chromatography tandem mass spectrometry JO: Biomed Chromatogr http://www.kidney.de/pget.php?&tw=1&pmid=29517154 #FOAvip Nephropathic cystinosis is characterized by abnormal intralysosomal accumulation of cystine throughout the body, causing irreversible damage to various organs, particularly the kidneys. Cysteamine, the currently available treatment, can reduce lysosomal cystine and postpone disease progression. However, cysteamine poses serious side effects and does not address all of the symptoms of cystinosis. To screen for new treatment options, a rapid and reliable high?performance liquid chromatography?tandem mass spectrometry (HPLC?MS/MS) method was developed to quantify cystine in conditionally immortalized human proximal tubular epithelial cells (ciPTEC). The ciPTEC were treated with N?ethylmaleimide, lysed and deproteinized with 15% (w/v) sulfosalicylic acid. Subsequently, cystine was measured using deuterium?labeled cystine?D4, as the internal standard. The assay developed demonstrated linearity to at least 20??mol/L with a good precision. Accuracies were between 97.3 and 102.9% for both cell extracts and whole cell samples. Cystine was sufficiently stable under all relevant analytical conditions. The assay was successfully applied to determine cystine levels in both healthy and cystinotic ciPTEC. Control cells showed clearly distinguishable cystine levels compared with cystinotic cells treated with or without cysteamine. The method developed provides a fast and reliable quantification of cystine, and is applicable to screen for potential drugs that could reverse cystinotic symptoms in human kidney cells. Twit Text FOAVip Quantification of cystine in human renal proximal tubule cells using liquid chromatography tandem mass spectrometry ????Biomed Chromatogr http://www.kidney.de/pget.php?&tw=1&pmid=29517154 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29517154 #FOAvip English Wikipedia <ref>{{Cite pmid|29517154}}</ref> Quantification of cystine in human renal proximal tubule cells using liquid chromatography?tandem mass spectrometry #MMPMID29517154 Jamalpoor A; Sparidans RW; Pou Casellas C; Rood JJ; Joshi M; Masereeuw R; Janssen MJ Biomed Chromatogr 2018[Aug]; 32 (8): � PMID29517154show ga Nephropathic cystinosis is characterized by abnormal intralysosomal accumulation of cystine throughout the body, causing irreversible damage to various organs, particularly the kidneys. Cysteamine, the currently available treatment, can reduce lysosomal cystine and postpone disease progression. However, cysteamine poses serious side effects and does not address all of the symptoms of cystinosis. To screen for new treatment options, a rapid and reliable high?performance liquid chromatography?tandem mass spectrometry (HPLC?MS/MS) method was developed to quantify cystine in conditionally immortalized human proximal tubular epithelial cells (ciPTEC). The ciPTEC were treated with N?ethylmaleimide, lysed and deproteinized with 15% (w/v) sulfosalicylic acid. Subsequently, cystine was measured using deuterium?labeled cystine?D4, as the internal standard. The assay developed demonstrated linearity to at least 20??mol/L with a good precision. Accuracies were between 97.3 and 102.9% for both cell extracts and whole cell samples. Cystine was sufficiently stable under all relevant analytical conditions. The assay was successfully applied to determine cystine levels in both healthy and cystinotic ciPTEC. Control cells showed clearly distinguishable cystine levels compared with cystinotic cells treated with or without cysteamine. The method developed provides a fast and reliable quantification of cystine, and is applicable to screen for potential drugs that could reverse cystinotic symptoms in human kidney cells. �Quantification of cystine in human renal proximal tubule cells using l(epmc).pdf DeepDyve Pubget Overpricing